FastQCFastQC Report
Thu 26 May 2016
SRR1512428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681826
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79290.4714518624399909No Hit
CTGTAGGACGTGGAATATGGCAAGA66010.39249006734347075No Hit
GTCCTACAGTGGACATTTCTAAATT61500.36567397578584226No Hit
GTCCTAAAGTGTGTATTTCTCATTT60710.3609767003245282No Hit
CTTTAGGACGTGAAATATGGCGAGG58610.3484902718830604No Hit
GGTATCAACGCAGAGTACTTTTTTT57270.3405227413537429No Hit
TATCAACGCAGAGTACTTTTTTTTT53380.3173931191455002No Hit
GTCCTACAGTGTGCATTTCTCATTT33550.19948555914821153No Hit
CTGAAGGACCTGGAATATGGCGAGA30760.18289644707597574No Hit
CTGTAGGACCTGGAATATGGCGAGA27060.1608965493457706No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25500.1516209167892517No Hit
ATTTAGAAATGTCCACTGTAGGACG25310.15049119231121413No Hit
GTCCTTCAGTGTGCATTTCTCATTT24180.14377230462604335No Hit
TTTCTAAATTTTCCACCTTTTTCAG24030.14288041688022424No Hit
GAATATGGCAAGAAAACTGAAAATC22980.13663720265949034No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20410.12135619261445596No Hit
GGAATATGGCGAGAAAACTGAAAAT19520.11606432532259581No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17800.10583734583720314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG551.1122458E-515.5691491
GGTATCA11900.013.8319761
TAGGACC10200.013.5162234
TGCGCAT500.001501601213.29768910
GTCCTAT2300.012.4101911
CCACCTT8000.012.10859413
GGCGAGG16700.011.65479719
TTCCACC8550.011.44245611
CCGGTTT1104.9854316E-711.2243212
CACCTTT8800.011.113420514
AGGACCT21350.011.0889145
GGACCTG20450.011.0546436
TTAGGCC700.001481120410.8662454
GTCGAGG700.001498872310.85073919
TCCAACG1408.427378E-910.85073918
TTAGGAC23250.010.7143513
AATGGCG803.7916377E-410.68119516
ATAGGAC3300.010.6604453
ACCTTTT9600.010.58292515
TCCACCT9350.010.46248812
Produced by FastQC (version 0.11.5)