##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512428_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1681826 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91074046899025 34.0 31.0 34.0 30.0 34.0 2 32.08244431944803 34.0 31.0 34.0 30.0 34.0 3 32.209013893232715 34.0 31.0 34.0 30.0 34.0 4 35.65361339401341 37.0 35.0 37.0 35.0 37.0 5 35.47093099999643 37.0 35.0 37.0 33.0 37.0 6 35.50090199580694 37.0 35.0 37.0 33.0 37.0 7 35.41435677650363 37.0 35.0 37.0 33.0 37.0 8 35.377512299132015 37.0 35.0 37.0 33.0 37.0 9 37.02248389548027 39.0 37.0 39.0 33.0 39.0 10 36.946883328001825 39.0 37.0 39.0 33.0 39.0 11 36.98907556429738 39.0 37.0 39.0 33.0 39.0 12 36.92280652100752 39.0 37.0 39.0 33.0 39.0 13 36.90011273461107 39.0 37.0 39.0 33.0 39.0 14 38.22591754438331 40.0 38.0 41.0 33.0 41.0 15 38.231424059325995 40.0 38.0 41.0 33.0 41.0 16 38.18906295895057 40.0 38.0 41.0 33.0 41.0 17 38.19091332872723 40.0 38.0 41.0 33.0 41.0 18 38.14773942131945 40.0 38.0 41.0 33.0 41.0 19 38.17824138763463 40.0 39.0 41.0 34.0 41.0 20 38.15926439477092 40.0 39.0 41.0 34.0 41.0 21 38.112197100056726 40.0 38.0 41.0 33.0 41.0 22 38.05684654655119 40.0 38.0 41.0 33.0 41.0 23 37.98965112918935 40.0 38.0 41.0 33.0 41.0 24 37.9467251665749 40.0 38.0 41.0 33.0 41.0 25 37.88908840748092 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4715654555294435 1101 2 0.4764592644261185 1101 3 0.527495788971919 1101 4 0.37499488846212614 1101 5 0.3070238168874013 1101 6 0.46220004396619885 1101 7 0.46696967879513807 1101 8 0.5677662672537451 1101 9 0.5983844237933837 1101 10 0.6254488110520384 1101 11 0.5010317313685917 1101 12 0.5990491526429196 1101 13 0.7370254174415791 1101 14 0.7211145878559293 1101 15 0.7167859843887499 1101 16 0.8500964832705336 1101 17 0.7130594058014736 1101 18 0.8026838139125161 1101 19 0.8066689202567687 1101 20 0.7713424912710494 1101 21 0.8121346234573892 1101 22 0.6623242147446788 1101 23 0.6832292064488854 1101 24 0.8194745097097709 1101 25 0.9007187444626936 1102 1 0.5114771912747855 1102 2 0.5014187663795582 1102 3 0.5083239109649256 1102 4 0.4455158605647185 1102 5 0.45922173509162434 1102 6 0.4674589044903641 1102 7 0.47965327461061946 1102 8 0.4930263983048633 1102 9 0.5976030497184865 1102 10 0.6518107505487976 1102 11 0.5929584668056478 1102 12 0.6037179655036411 1102 13 0.6013495811826104 1102 14 0.7927898710619914 1102 15 0.8228921742839503 1102 16 0.8451870176579064 1102 17 0.794172462147479 1102 18 0.7596854450449086 1102 19 0.7881315583565112 1102 20 0.8874499703774248 1102 21 0.8882313404531175 1102 22 0.9126658557049723 1102 23 0.8722821220279755 1102 24 0.836001823236586 1102 25 0.9011469867820736 1103 1 0.32497349701880296 1103 2 0.3195655534801176 1103 3 0.16904659277340528 1103 4 0.15654009646667788 1103 5 0.21914054594293475 1103 6 0.21557791204071464 1103 7 0.3202493461195246 1103 8 0.2572463518856054 1103 9 0.34112076849446993 1103 10 0.31305850970191074 1103 11 0.4578449798750128 1103 12 0.3208085736059445 1103 13 0.4094924507000002 1103 14 0.46982417148108624 1103 15 0.41180188486402614 1103 16 0.49529479575372903 1103 17 0.5639796949542273 1103 18 0.49534874819847374 1103 19 0.4740123248054573 1103 20 0.4091005882612109 1103 21 0.4418029536240411 1103 22 0.46526145952787346 1103 23 0.3583105375170845 1103 24 0.34500103579880204 1103 25 0.4733476169461852 1104 1 0.3737447792713269 1104 2 0.41264575140481696 1104 3 0.3101704687509539 1104 4 0.21529720133774077 1104 5 0.07761812272119073 1104 6 0.11974876004705948 1104 7 0.10338684628132455 1104 8 0.11069712380689367 1104 9 0.2390024047318846 1104 10 0.27044972163298553 1104 11 0.24137046748223412 1104 12 0.2693217198741422 1104 13 0.24472893228404757 1104 14 0.22995936415654228 1104 15 0.31222093855617317 1104 16 0.3646779195602221 1104 17 0.36817990601394257 1104 18 0.34908701915745866 1104 19 0.4151676757961411 1104 20 0.40000757959740696 1104 21 0.3392942179453371 1104 22 0.28803295025126374 1104 23 0.4207754943138795 1104 24 0.4503432039366899 1104 25 0.39092089343373715 1105 1 0.4137188936505183 1105 2 0.4286769530984458 1105 3 0.37698097301236544 1105 4 0.207490023481526 1105 5 0.2237657389475629 1105 6 0.2981105939377926 1105 7 0.3592090203003693 1105 8 0.39022098019830764 1105 9 0.5253694826479887 1105 10 0.49575593939255214 1105 11 0.46137251311529326 1105 12 0.5193881386203785 1105 13 0.5796801169178707 1105 14 0.6140359763308894 1105 15 0.53565295760243 1105 16 0.5648067687115059 1105 17 0.5546387157771449 1105 18 0.5320254745501174 1105 19 0.5516358452954933 1105 20 0.6612773773692098 1105 21 0.6022233586314982 1105 22 0.6441220214888261 1105 23 0.6479385422042085 1105 24 0.7097709670369454 1105 25 0.8267845076041809 1106 1 0.3649284586254211 1106 2 0.33013749409118986 1106 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0.7201212355828446 1107 16 0.8053317865208527 1107 17 0.7099177672177746 1107 18 0.7328444575831199 1107 19 0.8082799232979525 1107 20 0.7552616854042213 1107 21 0.8080523198326333 1107 22 0.7070395950747326 1107 23 0.8309866792563625 1107 24 0.7888362273824043 1107 25 0.8085536623143241 1108 1 0.29206268592228923 1108 2 0.26901313982092745 1108 3 0.20243483130769846 1108 4 0.18801705682859193 1108 5 0.283801536388431 1108 6 0.2126910758318843 1108 7 0.23768106254645005 1108 8 0.198141023299776 1108 9 0.24971348995320852 1108 10 0.20859506505353664 1108 11 0.23353125044838663 1108 12 0.04736247914745917 1108 13 0.07963494804701554 1108 14 0.20465494980387433 1108 15 0.2695413694619688 1108 16 0.4245069244182318 1108 17 0.32695265631240034 1108 18 0.34279782077528864 1108 19 0.2533219622209657 1108 20 0.3021386485130506 1108 21 0.2980452232676498 1108 22 0.277435796454256 1108 23 0.3714150109996268 1108 24 0.4764730085810527 1108 25 0.3876086129125156 1109 1 0.03537348833908638 1109 2 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-0.9668784873252179 2315 13 -1.0060643716761106 2315 14 -1.1397833325007696 2315 15 -1.121195883292593 2315 16 -1.2427004867005493 2315 17 -1.1550120730008686 2315 18 -1.1671883494475992 2315 19 -0.986318593713051 2315 20 -1.0553733530606308 2315 21 -1.0134143212556737 2315 22 -1.1376649887347199 2315 23 -1.1725990179903505 2315 24 -1.2370020895613152 2315 25 -1.1525355311053644 2316 1 -1.5407963312905473 2316 2 -1.2252435219515867 2316 3 -1.1926059359285652 2316 4 -0.938583174341943 2316 5 -1.0706188191143653 2316 6 -1.203854799023631 2316 7 -1.2683465529607219 2316 8 -1.2163336885180343 2316 9 -1.464795585052265 2316 10 -1.493193382667556 2316 11 -1.509494584420878 2316 12 -1.5282396576180304 2316 13 -1.4624880721647884 2316 14 -1.6067270751250575 2316 15 -1.8467920784153193 2316 16 -1.958859730794039 2316 17 -2.0623275911025516 2316 18 -2.23460057352667 2316 19 -2.1766349275981582 2316 20 -2.046680507402101 2316 21 -2.1631196887938273 2316 22 -2.0664241266971644 2316 23 -2.108743372056196 2316 24 -2.1555144332358225 2316 25 -2.1399491016231096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11640.0 3 0.0 4 233.0 5 1939.0 6 2523.0 7 1905.0 8 1635.0 9 1454.0 10 1382.0 11 1202.0 12 1192.0 13 1172.0 14 1185.0 15 1193.0 16 1236.0 17 1357.0 18 1427.0 19 1745.0 20 2106.0 21 2692.0 22 3673.0 23 4600.0 24 5606.0 25 6804.0 26 7531.0 27 8915.0 28 10466.0 29 12750.0 30 16278.0 31 21569.0 32 28398.0 33 37991.0 34 51924.0 35 71938.0 36 108393.0 37 203088.0 38 1042684.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.269550105623125 20.42525592838472 13.371465258120057 24.933728707872092 2 14.010834145993387 21.21589800918108 42.927479009585426 21.845788835240114 3 18.227747698037728 26.219180818943222 30.681057374544096 24.872014108474954 4 11.947044990352627 18.174699207101895 39.69336516421345 30.18489063833203 5 11.938846760334327 38.99700638902894 36.76807878512612 12.296068065510614 6 29.809259896995943 38.82262044747703 18.904259995134698 12.463859660392329 7 26.483219568536327 32.216025640644396 23.740315321878942 17.560439468940338 8 23.3948244238853 39.268518100005 21.327305945632187 16.00935153047752 9 26.05531555227224 14.915653864707146 21.59375265802893 37.43527792499169 10 14.9374587370412 29.307971854944714 35.629962707206964 20.124606700807117 11 32.690762335572785 22.145472173398407 27.17338353512192 17.99038195590689 12 23.59201842414553 24.932432110925536 33.258568512412026 18.216980952516902 13 29.12887837388568 22.962078782828904 26.406872339653635 21.502170503631778 14 19.370349183620803 24.26731617048048 28.746906393196387 27.615428252702333 15 21.354265774851573 34.2190830035013 23.81367502664028 20.612976195006848 16 19.857318096329905 28.656848118930924 30.81794738084521 20.667886403893963 17 20.00674272347758 30.252293209768265 29.510415248306444 20.230548818447712 18 19.70591383144451 27.32676505214589 33.540450226540294 19.426870889869306 19 22.81121828298528 26.901772240410125 29.10883765621414 21.178171820390457 20 22.948687914207532 27.953010596815602 30.050552197433028 19.047749291543834 21 21.309291989356797 27.1477115633315 28.731586223299093 22.811410224012604 22 22.358397726157808 29.859282229364315 28.73264812827229 19.049671916205586 23 20.25655677124243 29.499985432370224 30.130198934363015 20.11325886202433 24 22.485798174127407 27.29664067507578 30.822689148580174 19.394872002216637 25 21.08999385192047 28.637147957041933 30.29766456220798 19.97519362882962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 341.0 1 341.0 2 587.0 3 833.0 4 833.0 5 833.0 6 1941.0 7 3049.0 8 3049.0 9 3049.0 10 4122.5 11 5196.0 12 5196.0 13 5196.0 14 7577.5 15 9959.0 16 9959.0 17 9959.0 18 17750.0 19 25541.0 20 25541.0 21 25541.0 22 40730.5 23 55920.0 24 55920.0 25 55920.0 26 81462.0 27 107004.0 28 107004.0 29 107004.0 30 136763.5 31 166523.0 32 166523.0 33 166523.0 34 181564.5 35 196606.0 36 196606.0 37 196606.0 38 201372.0 39 206138.0 40 206138.0 41 206138.0 42 208580.5 43 211023.0 44 211023.0 45 211023.0 46 212250.0 47 213477.0 48 213477.0 49 213477.0 50 196841.5 51 180206.0 52 180206.0 53 180206.0 54 151864.5 55 123523.0 56 123523.0 57 123523.0 58 103047.0 59 82571.0 60 82571.0 61 82571.0 62 66868.0 63 51165.0 64 51165.0 65 51165.0 66 38285.0 67 25405.0 68 25405.0 69 25405.0 70 18296.5 71 11188.0 72 11188.0 73 11188.0 74 7629.5 75 4071.0 76 4071.0 77 4071.0 78 2763.5 79 1456.0 80 1456.0 81 1456.0 82 947.5 83 439.0 84 439.0 85 439.0 86 298.5 87 158.0 88 158.0 89 158.0 90 93.0 91 28.0 92 28.0 93 28.0 94 17.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16309653911879113 2 0.006302673403788502 3 0.0 4 0.0012486428441467786 5 0.11386433554957529 6 0.031691744568106334 7 0.025745826262645485 8 0.023189081391297314 9 0.0356160506497105 10 0.03187012211727016 11 0.036329560846365794 12 0.03294038741225311 13 0.024913397699880963 14 0.007848612163208323 15 0.009632387654846577 16 0.0025567448713481656 17 7.729693797099105E-4 18 3.5675509832765103E-4 19 0.0 20 0.0 21 5.94591830546085E-5 22 0.005410785657969374 23 8.918877458191275E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1681826.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.66917601762957 #Duplication Level Percentage of deduplicated Percentage of total 1 84.14033780889999 56.095669915643654 2 11.214456011790293 14.953170835840224 3 2.455307243385703 4.910799323599268 4 0.8195106759002018 2.185444059996685 5 0.39771053952003643 1.3257516981663866 6 0.2029925041352892 0.8120005793072995 7 0.13280424254960485 0.6197764595699303 8 0.09621611001287722 0.5131719019344098 9 0.07299854134070019 0.4380077341506015 >10 0.39441928789008357 4.939477085886262 >50 0.03590279212789277 1.6397126608636472 >100 0.030303610812975706 4.205507780443265 >500 0.0037431206157445647 1.7081750013901713 >1k 0.002673657582674689 3.0581852350186827 >5k 6.238534359574274E-4 2.5951497281894502 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7929 0.4714518624399909 No Hit CTGTAGGACGTGGAATATGGCAAGA 6601 0.39249006734347075 No Hit GTCCTACAGTGGACATTTCTAAATT 6150 0.36567397578584226 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6071 0.3609767003245282 No Hit CTTTAGGACGTGAAATATGGCGAGG 5861 0.3484902718830604 No Hit GGTATCAACGCAGAGTACTTTTTTT 5727 0.3405227413537429 No Hit TATCAACGCAGAGTACTTTTTTTTT 5338 0.3173931191455002 No Hit GTCCTACAGTGTGCATTTCTCATTT 3355 0.19948555914821153 No Hit CTGAAGGACCTGGAATATGGCGAGA 3076 0.18289644707597574 No Hit CTGTAGGACCTGGAATATGGCGAGA 2706 0.1608965493457706 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2550 0.1516209167892517 No Hit ATTTAGAAATGTCCACTGTAGGACG 2531 0.15049119231121413 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2418 0.14377230462604335 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2403 0.14288041688022424 No Hit GAATATGGCAAGAAAACTGAAAATC 2298 0.13663720265949034 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2041 0.12135619261445596 No Hit GGAATATGGCGAGAAAACTGAAAAT 1952 0.11606432532259581 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1780 0.10583734583720314 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.94591830546085E-5 0.0 8 0.0 0.0 0.0 5.94591830546085E-5 0.0 9 0.0 0.0 0.0 5.94591830546085E-5 0.0 10 0.0 0.0 0.0 1.18918366109217E-4 0.0 11 0.0 0.0 0.0 1.18918366109217E-4 5.94591830546085E-5 12 0.0 0.0 0.0 1.18918366109217E-4 1.18918366109217E-4 13 0.0 0.0 0.0 1.7837754916382551E-4 1.18918366109217E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 55 1.1122458E-5 15.569149 1 GGTATCA 1190 0.0 13.831976 1 TAGGACC 1020 0.0 13.516223 4 TGCGCAT 50 0.0015016012 13.297689 10 GTCCTAT 230 0.0 12.410191 1 CCACCTT 800 0.0 12.108594 13 GGCGAGG 1670 0.0 11.654797 19 TTCCACC 855 0.0 11.442456 11 CCGGTTT 110 4.9854316E-7 11.22432 12 CACCTTT 880 0.0 11.1134205 14 AGGACCT 2135 0.0 11.088914 5 GGACCTG 2045 0.0 11.054643 6 TTAGGCC 70 0.0014811204 10.866245 4 GTCGAGG 70 0.0014988723 10.850739 19 TCCAACG 140 8.427378E-9 10.850739 18 TTAGGAC 2325 0.0 10.714351 3 AATGGCG 80 3.7916377E-4 10.681195 16 ATAGGAC 330 0.0 10.660445 3 ACCTTTT 960 0.0 10.582925 15 TCCACCT 935 0.0 10.462488 12 >>END_MODULE