FastQCFastQC Report
Thu 26 May 2016
SRR1512427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169920
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62990.2902871995280932No Hit
GTATCAACGCAGAGTACTTTTTTTT60940.28083984663029055No Hit
GTCCTAAAGTGTGTATTTCTCATTT60030.27664614363663176No Hit
CTGTAGGACGTGGAATATGGCAAGA58250.2684430762424421No Hit
CTTTAGGACGTGAAATATGGCGAGG47420.2185334021530748No Hit
GTCCTACAGTGTGCATTTCTCATTT40510.18668891019023742No Hit
GGTATCAACGCAGAGTACTTTTTTT39500.18203436071375903No Hit
TATCAACGCAGAGTACTTTTTTTTT34920.16092759180061936No Hit
CTGTAGGACCTGGAATATGGCGAGA28220.1300508774517033No Hit
CTGAAGGACCTGGAATATGGCGAGA26840.12369119598879222No Hit
GTCCTTCAGTGTGCATTTCTCATTT24360.11226220321486508No Hit
ATTTAGAAATGTCCACTGTAGGACG24250.11175527208376346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCCC350.002245166616.2035268
GGTATCA13000.013.36531451
TAGGACC12350.012.9775614
ACGTTAG550.003108531512.0703197
GTATAGG2400.011.5174591
GGACCTG21500.011.5129196
AATGTCC9050.011.4886888
GGCGAGG16450.011.40592719
TAAGACG752.1080748E-411.3803234
GTCCTAC34650.011.31091
AGGACCT22450.011.152215
TAGAAAT10250.011.1027544
TACGGCA600.005925290311.07113216
TGTAGGA38400.011.0145532
GTGTAGG4500.010.9690081
ATTTAGA9500.010.9112771
GATATAC4350.010.893361
CTAGGAC3150.010.8384033
TCCTACA40100.010.8077462
CCTACAG39800.010.794153
Produced by FastQC (version 0.11.5)