FastQCFastQC Report
Thu 26 May 2016
SRR1512427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169920
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73790.34005861967261464No Hit
CTGTAGGACGTGGAATATGGCAAGA67690.31194698422061645No Hit
GTCCTACAGTGGACATTTCTAAATT66030.30429693260581037No Hit
GTCCTAAAGTGTGTATTTCTCATTT62210.2866925969621No Hit
CTTTAGGACGTGAAATATGGCGAGG57400.26452588113847514No Hit
GGTATCAACGCAGAGTACTTTTTTT51440.23705943076242444No Hit
TATCAACGCAGAGTACTTTTTTTTT49540.2283033475888512No Hit
GTCCTACAGTGTGCATTTCTCATTT44110.2032793835717446No Hit
CTGTAGGACCTGGAATATGGCGAGA32970.15194108538563633No Hit
CTGAAGGACCTGGAATATGGCGAGA32250.1486229907093349No Hit
ATTTAGAAATGTCCACTGTAGGACG26820.12359902669222829No Hit
GTCCTTCAGTGTGCATTTCTCATTT25210.11617939831883205No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23970.11046490193186845No Hit
GAATATGGCAAGAAAACTGAAAATC23570.10862151600058989No Hit
TTTCTAAATTTTCCACCTTTTTCAG23130.10659379147618345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.014.0909821
TAGGACC11500.013.1437464
CGCATTT658.0387515E-411.69003812
TCCAACG1552.5465852E-1111.63887618
AATCCCG1800.011.60484519
AATGTCC9100.011.5864648
CCCCCGG752.0849644E-411.39384718
AGGACCT23050.011.2595655
GCCGGTT1104.980575E-711.22561111
GGACCTG22000.011.1395176
GGCGAGG15350.011.01034119
CCCTATA951.3498302E-511.0080152
TAGAAAT9950.010.9873914
CCAACGT1053.4901732E-610.85128319
GAAATGT9650.010.8276626
ATTAGAC1502.1100277E-910.77401353
AGGACGT37700.010.6162385
TTAGACT2150.010.6118884
GACGTGG18900.010.6044947
TGTAGGA39100.010.60106852
Produced by FastQC (version 0.11.5)