FastQCFastQC Report
Thu 26 May 2016
SRR1512426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591792
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60990.3831530752761667No Hit
GTCCTAAAGTGTGTATTTCTCATTT58290.36619106013851055No Hit
CTGTAGGACGTGGAATATGGCAAGA55180.34665333159106215No Hit
GTATCAACGCAGAGTACTTTTTTTT50620.3180063726919095No Hit
CTTTAGGACGTGAAATATGGCGAGG47990.3014841135022666No Hit
GTCCTACAGTGTGCATTTCTCATTT37800.23746821192718648No Hit
GGTATCAACGCAGAGTACTTTTTTT31880.2002774231809181No Hit
TATCAACGCAGAGTACTTTTTTTTT28320.177912692110527No Hit
CTGTAGGACCTGGAATATGGCGAGA24570.1543543377526712No Hit
CTGAAGGACCTGGAATATGGCGAGA24220.15215555801260466No Hit
GTCCTTCAGTGTGCATTTCTCATTT23550.1479464653673344No Hit
ATTTAGAAATGTCCACTGTAGGACG20460.12853438137646125No Hit
GAATATGGCAAGAAAACTGAAAATC19860.12476504467920431No Hit
TTTCTAAATTTTCCACCTTTTTCAG18050.11339421230914591No Hit
GGAATATGGCGAGAAAACTGAAAAT16220.1018977353825123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA1900.014.5286091
GTGCGAG400.0053583814.21804211
TAGGACC10100.013.98959354
TCCAACG1900.012.97166218
GGTATCA9300.012.7209031
CCAACGA1052.0489097E-812.64184519
GTCTAAT1102.2746462E-812.5474361
CCACCTT7250.012.16424513
CACCGTG550.003110224412.06911857
ACCTTTT7800.012.04127615
CACCTTT7700.011.9512414
GTGCTAG1509.276846E-1111.830441
GGCGAGG15000.011.63049719
CGCGCCT1157.290146E-811.54000812
AGGACCT21250.011.5133315
GGACCTG21050.011.4425236
CCAACGT1001.9755316E-611.37766119
GTCCTAA16050.011.2407611
GTCGAGG855.4301883E-511.15456919
GCGCCTT1201.3072349E-711.06335913
Produced by FastQC (version 0.11.5)