FastQCFastQC Report
Thu 26 May 2016
SRR1512426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591792
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65700.41274236834963357No Hit
CTGTAGGACGTGGAATATGGCAAGA62130.3903148150009549No Hit
GTCCTACAGTGGACATTTCTAAATT54460.34213012755435385No Hit
CTTTAGGACGTGAAATATGGCGAGG53310.33490556555127804No Hit
GTCCTAAAGTGTGTATTTCTCATTT52800.3317016293586097No Hit
TATCAACGCAGAGTACTTTTTTTTT47190.2964583312392574No Hit
GGTATCAACGCAGAGTACTTTTTTT45910.28841707961844265No Hit
GTCCTACAGTGTGCATTTCTCATTT33000.20731351834913106No Hit
CTGAAGGACCTGGAATATGGCGAGA29600.18595394373134178No Hit
CTGTAGGACCTGGAATATGGCGAGA29180.18331540804326193No Hit
TTTCTAAATTTTCCACCTTTTTCAG23920.1502708896639762No Hit
ATTTAGAAATGTCCACTGTAGGACG23550.1479464653673344No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23360.14675284207986974No Hit
GAATATGGCAAGAAAACTGAAAATC22860.14361172816548895No Hit
GGAATATGGCGAGAAAACTGAAAAT19820.12451375556605385No Hit
GTCCTTCAGTGTGCATTTCTCATTT18670.11728919356297808No Hit
ATTCCAGGTCCTTCAGTGTGCATTT16120.10126951259963614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT250.005998566319.018371
TACGGCG508.7334665E-515.19605516
ATTGCGA851.722583E-814.525640517
TATTGCG954.9185473E-913.99636516
TAGGACC10700.013.5003064
GGTATCA12850.013.4682621
TCCAACG1251.03682396E-1012.91664618
TACGCTG604.079409E-412.6713395
ACGGCGA604.1010953E-412.66337917
ATACGGC905.42228E-712.66337915
ACCTTTT9250.012.62915315
CCACCTT9000.012.34795813
TTCCACC8650.012.29931711
GCGCCAC853.9507795E-612.29208513
ATTAGGC550.003060034712.0953693
CTGTCCG550.003070857612.0896699
GCGTGCG550.003070857612.0896699
TCCACCT8900.011.95307912
AAGGCGT802.8694702E-511.8737836
TTGCGAG802.8740867E-511.87191818
Produced by FastQC (version 0.11.5)