##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512426_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1591792 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.706984957833686 31.0 31.0 34.0 27.0 34.0 2 30.9249751223778 31.0 31.0 34.0 27.0 34.0 3 31.016443103119002 31.0 31.0 34.0 27.0 34.0 4 34.597160307376846 37.0 35.0 37.0 32.0 37.0 5 34.308485656417425 37.0 35.0 37.0 30.0 37.0 6 34.35191783851156 37.0 35.0 37.0 30.0 37.0 7 34.23825851618805 37.0 35.0 37.0 30.0 37.0 8 34.17688115030104 37.0 35.0 37.0 30.0 37.0 9 35.66923567903344 39.0 35.0 39.0 30.0 39.0 10 35.4902631750882 39.0 35.0 39.0 30.0 39.0 11 35.569143455928916 39.0 35.0 39.0 30.0 39.0 12 35.4388318322997 39.0 35.0 39.0 30.0 39.0 13 35.42869985525747 39.0 35.0 39.0 30.0 39.0 14 36.49514195322002 40.0 36.0 41.0 30.0 41.0 15 36.47665901072502 40.0 36.0 41.0 30.0 41.0 16 36.40337431021138 40.0 36.0 41.0 30.0 41.0 17 36.37915255259481 40.0 36.0 41.0 30.0 41.0 18 36.30042367344477 40.0 36.0 41.0 29.0 41.0 19 36.32984271814408 40.0 36.0 41.0 29.0 41.0 20 36.29448005769598 40.0 36.0 41.0 29.0 41.0 21 36.226741936132356 40.0 36.0 41.0 29.0 41.0 22 36.13792317086654 40.0 36.0 41.0 29.0 41.0 23 36.02094808869501 40.0 36.0 41.0 29.0 41.0 24 35.958336893262434 40.0 36.0 41.0 29.0 41.0 25 35.84632728396675 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5508074861181811 1101 2 0.5389424462738894 1101 3 0.6708965150857509 1101 4 0.6240933847983072 1101 5 0.4634302005052149 1101 6 0.6113408477527855 1101 7 0.6729811036246289 1101 8 0.8742586217672752 1101 9 0.8975599704322264 1101 10 0.9172621353206836 1101 11 0.8074130487895133 1101 12 0.794135784450468 1101 13 0.7710455465892281 1101 14 0.8599026738261699 1101 15 0.8248530803784817 1101 16 0.9774980944727503 1101 17 1.0504494397266484 1101 18 1.0801834995872355 1101 19 1.1311938431205064 1101 20 1.046803149282539 1101 21 1.0808289836970033 1101 22 1.0759447902649057 1101 23 1.0095951958949954 1101 24 1.130569243016403 1101 25 1.1554395345547164 1102 1 0.6206496354258704 1102 2 0.6712852298121739 1102 3 0.6349520459485944 1102 4 0.6036271864136822 1102 5 0.5163426212094819 1102 6 0.7454909347861403 1102 7 0.7616272597813278 1102 8 0.7961572679978488 1102 9 0.8872336816249273 1102 10 0.7422807901564994 1102 11 0.8378405574167402 1102 12 0.9533990351342538 1102 13 0.9464821847544158 1102 14 1.1636581983974494 1102 15 1.1423932263293182 1102 16 1.242603099380851 1102 17 1.276853863036834 1102 18 1.2407832009745619 1102 19 1.3103793760331826 1102 20 1.1959082245479564 1102 21 1.2022416909043656 1102 22 1.3096565647643033 1102 23 1.3380207683430712 1102 24 1.2876575055898982 1102 25 1.3065501315162322 1103 1 0.8278385535056394 1103 2 0.8854039667396023 1103 3 0.7623521516147633 1103 4 0.7875478354645011 1103 5 0.7633879491002844 1103 6 0.8746316372490313 1103 7 0.8582177165341918 1103 8 0.970166285809114 1103 9 0.889455566101411 1103 10 0.9512175424417109 1103 11 1.110263858977845 1103 12 1.0750743505043374 1103 13 1.1614404610931572 1103 14 1.21841643275922 1103 15 1.3026005057150059 1103 16 1.312270395702079 1103 17 1.295092133006655 1103 18 1.2711477492516963 1103 19 1.2245039028875837 1103 20 1.3019905428347158 1103 21 1.130428502898198 1103 22 1.177070855751559 1103 23 1.1607947223565773 1103 24 1.2551181159929499 1103 25 1.2071949846330199 1104 1 0.5556330236123905 1104 2 0.4251732254065992 1104 3 0.5501980280717369 1104 4 0.48840172553002503 1104 5 0.3817128997008794 1104 6 0.36879695841933113 1104 7 0.40542205258175557 1104 8 0.5281303981622543 1104 9 0.32521472935109585 1104 10 0.6029227412847789 1104 11 0.45860415533552157 1104 12 0.6599732350007343 1104 13 0.5348111843829528 1104 14 0.46651337819208294 1104 15 0.5368569076318721 1104 16 0.569857123751774 1104 17 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17 1.1369275836414587 1107 18 1.179150645456815 1107 19 1.143199461910669 1107 20 1.0238525678293229 1107 21 1.1231095834769746 1107 22 1.037014364264003 1107 23 1.1194734642884399 1107 24 1.089012556802075 1107 25 1.1606824337655155 1108 1 0.18082817879183466 1108 2 0.07814878253113022 1108 3 0.08324886288841782 1108 4 0.1865177943837537 1108 5 0.08669793366036771 1108 6 -0.023249908656374885 1108 7 0.07030083262570486 1108 8 -0.012530245626095393 1108 9 -0.005582000991680047 1108 10 -0.20222413474072454 1108 11 0.02113709554096488 1108 12 0.00807413892969322 1108 13 0.15813807055155138 1108 14 -0.001678813611896146 1108 15 0.07730499386808276 1108 16 0.20806925370274598 1108 17 0.2006675026955662 1108 18 0.19464745833656139 1108 19 0.11432892561926877 1108 20 0.22964681211885818 1108 21 0.08680823404727533 1108 22 -0.024424712515269675 1108 23 -0.09157132444675398 1108 24 0.09787533660736614 1108 25 0.14214144869177403 1109 1 -0.015230404391420649 1109 2 -0.08974747825788754 1109 3 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-1.7148904593587027 2316 3 -1.5272728747894284 2316 4 -1.520861179561578 2316 5 -1.495876128128188 2316 6 -1.6408162862509812 2316 7 -1.6211578994345643 2316 8 -1.8531625688776998 2316 9 -2.2092059814041463 2316 10 -2.145708916695753 2316 11 -2.3265649247815787 2316 12 -2.2286903142754753 2316 13 -2.198504581020373 2316 14 -2.199269389258255 2316 15 -2.3677577922010897 2316 16 -2.5256626872482784 2316 17 -2.7647070898273824 2316 18 -2.8512280147663773 2316 19 -3.026919445665712 2316 20 -2.684713204299541 2316 21 -3.061667336420598 2316 22 -3.1757359175973363 2316 23 -3.0232804371371316 2316 24 -2.9813482257085795 2316 25 -2.8930161250120534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27934.0 3 3.0 4 588.0 5 4048.0 6 4583.0 7 3711.0 8 3032.0 9 2747.0 10 2524.0 11 2402.0 12 2232.0 13 2135.0 14 2110.0 15 2265.0 16 2198.0 17 2223.0 18 2536.0 19 2870.0 20 3685.0 21 4723.0 22 5790.0 23 7292.0 24 8652.0 25 10096.0 26 11625.0 27 13245.0 28 15414.0 29 18391.0 30 23206.0 31 30280.0 32 39299.0 33 51874.0 34 70140.0 35 99100.0 36 160003.0 37 319560.0 38 629276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.606457796892236 21.569240367960333 14.465198364229407 23.359103470918026 2 15.763725369473603 22.335404881750858 41.03877305361985 20.862096695155692 3 20.1454712676028 26.220197111180354 30.74289856966237 22.891433051554475 4 13.823760931937246 19.065750879046803 38.2383631919215 28.87212499709445 5 12.318857890038599 39.44032337151263 36.15946037693149 12.081358361517282 6 30.595477385355945 38.95720613026724 18.090270076336164 12.35704640804065 7 27.00678011041948 32.114262233383826 23.190918132884132 17.68803952331256 8 23.72457522491419 39.7081691648755 20.68531993136497 15.881935678845343 9 26.08658497735123 14.839359915349077 21.13106252870761 37.94299257859209 10 15.103508552564199 29.65351794615306 35.17487469455128 20.068098806731467 11 33.48608547962352 21.76199694998388 26.800555713690578 17.951361856702025 12 23.95436832323192 24.83396438386806 32.81171655379806 18.39995073910197 13 29.615748789417367 22.713275851994453 26.148647672231967 21.522327686356213 14 19.57485278605493 24.301656498491266 28.08181784484941 28.041672870604394 15 21.616091302662472 34.616580711269656 23.238427434022505 20.52890055204537 16 20.235725158612837 28.814545326614606 30.447696870588636 20.502032644183924 17 20.307980710999203 30.464798076125714 29.11223102573961 20.114990187135472 18 20.132279004665826 27.28964365207154 33.19878853138014 19.379288811882496 19 23.436981716204127 26.707697990692253 28.62905454984068 21.22626574326294 20 23.481899645179773 27.938512066903215 29.507624111692987 19.07196417622403 21 21.677141234533153 27.034499482344426 28.323361343693144 22.96499793942927 22 22.804706278514285 29.756443553179967 28.328074956682787 19.11077521162296 23 20.543502024138892 29.58855073665589 29.714823833324537 20.15312340588068 24 23.05992240192186 27.177482987727043 30.264004342275875 19.498590268075226 25 21.549612009609294 28.74452189733332 29.81758923276408 19.888276860293306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 228.0 1 228.0 2 402.5 3 577.0 4 577.0 5 577.0 6 1382.0 7 2187.0 8 2187.0 9 2187.0 10 3086.5 11 3986.0 12 3986.0 13 3986.0 14 5930.0 15 7874.0 16 7874.0 17 7874.0 18 14775.5 19 21677.0 20 21677.0 21 21677.0 22 35707.5 23 49738.0 24 49738.0 25 49738.0 26 74467.0 27 99196.0 28 99196.0 29 99196.0 30 127770.5 31 156345.0 32 156345.0 33 156345.0 34 170397.5 35 184450.0 36 184450.0 37 184450.0 38 189452.0 39 194454.0 40 194454.0 41 194454.0 42 198436.5 43 202419.0 44 202419.0 45 202419.0 46 202203.5 47 201988.0 48 201988.0 49 201988.0 50 186721.0 51 171454.0 52 171454.0 53 171454.0 54 145431.0 55 119408.0 56 119408.0 57 119408.0 58 99936.5 59 80465.0 60 80465.0 61 80465.0 62 65374.5 63 50284.0 64 50284.0 65 50284.0 66 38005.5 67 25727.0 68 25727.0 69 25727.0 70 18870.0 71 12013.0 72 12013.0 73 12013.0 74 8319.5 75 4626.0 76 4626.0 77 4626.0 78 3234.0 79 1842.0 80 1842.0 81 1842.0 82 1240.0 83 638.0 84 638.0 85 638.0 86 398.5 87 159.0 88 159.0 89 159.0 90 101.0 91 43.0 92 43.0 93 43.0 94 27.0 95 11.0 96 11.0 97 11.0 98 7.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11546734749263722 2 0.004271914923557852 3 0.0 4 6.910450611637702E-4 5 0.06608903675857147 6 0.017527415642244717 7 0.01413501261471348 8 0.011622121483208861 9 0.019789017660598873 10 0.017653060198819945 11 0.020417240443475027 12 0.016710726024505712 13 0.012752922492385941 14 0.0038321589755445436 15 0.00471167087157116 16 0.0014449124006151557 17 2.512891131504619E-4 18 3.141113914380773E-4 19 0.0 20 0.0 21 0.0 22 0.0028898248012303113 23 2.512891131504619E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1591792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.29375660084092 #Duplication Level Percentage of deduplicated Percentage of total 1 87.12140549959587 60.36969467412161 2 9.069858779012725 12.56969173273816 3 1.876870036226929 3.9016612638516093 4 0.6797994250165732 1.8842342357796014 5 0.32384455121676436 1.122020275426152 6 0.18885445309871385 0.7851860703604323 7 0.1242349832135768 0.6026096081677804 8 0.08955702336177715 0.49645940629814483 9 0.07468360186661913 0.4657596596837653 >10 0.38230547002248666 4.880961595005647 >50 0.032878022279564614 1.592107410443257 >100 0.028494686050503377 4.067816981275115 >500 0.0037870712449143817 1.7454080191795376 >1k 0.0029755559781470143 3.714435701886677 >5k 4.508418148707597E-4 1.8019533657826337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6570 0.41274236834963357 No Hit CTGTAGGACGTGGAATATGGCAAGA 6213 0.3903148150009549 No Hit GTCCTACAGTGGACATTTCTAAATT 5446 0.34213012755435385 No Hit CTTTAGGACGTGAAATATGGCGAGG 5331 0.33490556555127804 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5280 0.3317016293586097 No Hit TATCAACGCAGAGTACTTTTTTTTT 4719 0.2964583312392574 No Hit GGTATCAACGCAGAGTACTTTTTTT 4591 0.28841707961844265 No Hit GTCCTACAGTGTGCATTTCTCATTT 3300 0.20731351834913106 No Hit CTGAAGGACCTGGAATATGGCGAGA 2960 0.18595394373134178 No Hit CTGTAGGACCTGGAATATGGCGAGA 2918 0.18331540804326193 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2392 0.1502708896639762 No Hit ATTTAGAAATGTCCACTGTAGGACG 2355 0.1479464653673344 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2336 0.14675284207986974 No Hit GAATATGGCAAGAAAACTGAAAATC 2286 0.14361172816548895 No Hit GGAATATGGCGAGAAAACTGAAAAT 1982 0.12451375556605385 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1867 0.11728919356297808 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1612 0.10126951259963614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.282227828761546E-5 0.0 8 0.0 6.282227828761546E-5 0.0 6.282227828761546E-5 0.0 9 0.0 6.282227828761546E-5 0.0 1.2564455657523092E-4 0.0 10 0.0 6.282227828761546E-5 0.0 1.884668348628464E-4 0.0 11 0.0 6.282227828761546E-5 0.0 1.884668348628464E-4 6.282227828761546E-5 12 0.0 6.282227828761546E-5 0.0 1.884668348628464E-4 1.2564455657523092E-4 13 0.0 6.282227828761546E-5 0.0 1.884668348628464E-4 1.2564455657523092E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTAT 25 0.0059985663 19.01837 1 TACGGCG 50 8.7334665E-5 15.196055 16 ATTGCGA 85 1.722583E-8 14.5256405 17 TATTGCG 95 4.9185473E-9 13.996365 16 TAGGACC 1070 0.0 13.500306 4 GGTATCA 1285 0.0 13.468262 1 TCCAACG 125 1.03682396E-10 12.916646 18 TACGCTG 60 4.079409E-4 12.671339 5 ACGGCGA 60 4.1010953E-4 12.663379 17 ATACGGC 90 5.42228E-7 12.663379 15 ACCTTTT 925 0.0 12.629153 15 CCACCTT 900 0.0 12.347958 13 TTCCACC 865 0.0 12.299317 11 GCGCCAC 85 3.9507795E-6 12.292085 13 ATTAGGC 55 0.0030600347 12.095369 3 CTGTCCG 55 0.0030708576 12.089669 9 GCGTGCG 55 0.0030708576 12.089669 9 TCCACCT 890 0.0 11.953079 12 AAGGCGT 80 2.8694702E-5 11.873783 6 TTGCGAG 80 2.8740867E-5 11.871918 18 >>END_MODULE