FastQCFastQC Report
Thu 26 May 2016
SRR1512425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272581
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74010.3256649597968125No Hit
GTCCTACAGTGGACATTTCTAAATT70690.31105601956541923No Hit
GTCCTAAAGTGTGTATTTCTCATTT70100.30845985247610536No Hit
CTGTAGGACGTGGAATATGGCAAGA64520.2839062722076793No Hit
CTTTAGGACGTGAAATATGGCGAGG55560.2444797347157263No Hit
GGTATCAACGCAGAGTACTTTTTTT46130.20298506411872666No Hit
TATCAACGCAGAGTACTTTTTTTTT42660.18771608140699936No Hit
GTCCTACAGTGTGCATTTCTCATTT35670.15695810182343337No Hit
CTGAAGGACCTGGAATATGGCGAGA30980.13632077360498923No Hit
GTCCTTCAGTGTGCATTTCTCATTT26820.11801559548372534No Hit
ATTTAGAAATGTCCACTGTAGGACG25640.1128232613050976No Hit
CTGTAGGACCTGGAATATGGCGAGA24640.10842297810287069No Hit
TTTCTAAATTTTCCACCTTTTTCAG23890.10512276570120052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.014.6174381
ACCGTCT500.001557295113.2338938
TAGGACC9650.012.580034
AATCCCG1800.012.10846919
TCTATAC2050.011.5663093
AAGTCGT2650.011.4528287
GTGTAGG4950.010.96307851
TAGAAAT11200.010.9237244
AAATGTC10800.010.8894667
GGCGAGG18750.010.86603519
GTCCTAA20300.010.8388671
AGGACCT23400.010.8219135
GTGCGCC803.8503236E-410.66378411
AATGTCC10550.010.6623788
GGACCTG22450.010.6461576
GCGTGCG803.9385498E-410.6376649
GTCTACG650.00803927810.6257531
ACCTTTT10050.010.57573615
CACCTTT10550.010.52427214
CCAACGA1002.4670573E-510.42381319
Produced by FastQC (version 0.11.5)