FastQCFastQC Report
Thu 26 May 2016
SRR1512425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272581
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89930.3957174683762647No Hit
CTGTAGGACGTGGAATATGGCAAGA76310.3357856111619344No Hit
GTCCTACAGTGGACATTTCTAAATT74690.3286571523743268No Hit
GTCCTAAAGTGTGTATTTCTCATTT71820.3160283395839356No Hit
CTTTAGGACGTGAAATATGGCGAGG67200.29569903118964735No Hit
GGTATCAACGCAGAGTACTTTTTTT61320.26982536596055323No Hit
TATCAACGCAGAGTACTTTTTTTTT57670.25376433227242506No Hit
GTCCTACAGTGTGCATTTCTCATTT39570.17411920631211825No Hit
CTGAAGGACCTGGAATATGGCGAGA37930.16690274186046614No Hit
CTGTAGGACCTGGAATATGGCGAGA29840.13130445075445055No Hit
ATTTAGAAATGTCCACTGTAGGACG29600.1302483827859161No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28510.12545207409548878No Hit
TTTCTAAATTTTCCACCTTTTTCAG28250.1243080004629098No Hit
GTCCTTCAGTGTGCATTTCTCATTT28200.12408798630279844No Hit
GAATATGGCAAGAAAACTGAAAATC26830.1180595983157476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.013.6211331
TAGGACC11600.012.4578754
ACCTTTT9950.011.64183315
CACCTTT10150.011.50598214
GGCGAGG16600.011.26764719
CGGTTTC855.3442753E-511.17253413
GTCTAAC951.3348432E-511.0191651
TAGAAAT10650.010.980294
AATGTCC10850.010.9434968
GAAATGT10700.010.8305846
ATTTAGA10300.010.8100621
TGGTATC4650.010.6313792
AGGACCT24300.010.5639295
GTGGTAT5050.010.5530071
GGACCTG24000.010.52800756
CCACCTT10750.010.51257613
CCCGTAC1002.4149127E-510.44402216
ATAGTAC2100.010.4125573
TAGGACA11150.010.4026344
TGTCCAC11250.010.38506610
Produced by FastQC (version 0.11.5)