##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512425_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2272581 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96302882053489 34.0 31.0 34.0 30.0 34.0 2 32.13508033377028 34.0 31.0 34.0 30.0 34.0 3 32.260740541261235 34.0 31.0 34.0 30.0 34.0 4 35.69808292861729 37.0 35.0 37.0 35.0 37.0 5 35.52192507109758 37.0 35.0 37.0 33.0 37.0 6 35.55178539290789 37.0 35.0 37.0 33.0 37.0 7 35.46736507961653 37.0 35.0 37.0 33.0 37.0 8 35.43403425444462 37.0 35.0 37.0 33.0 37.0 9 37.09465405193478 39.0 38.0 39.0 34.0 39.0 10 37.02282558905491 39.0 37.0 39.0 34.0 39.0 11 37.059284135526966 39.0 37.0 39.0 34.0 39.0 12 36.99331288961758 39.0 37.0 39.0 33.0 39.0 13 36.9697788549671 39.0 37.0 39.0 33.0 39.0 14 38.31303702706306 41.0 38.0 41.0 34.0 41.0 15 38.3169457986316 41.0 38.0 41.0 33.0 41.0 16 38.27656879996797 41.0 38.0 41.0 33.0 41.0 17 38.273760539228306 41.0 38.0 41.0 33.0 41.0 18 38.2311169546872 41.0 38.0 41.0 33.0 41.0 19 38.253541677942394 41.0 39.0 41.0 34.0 41.0 20 38.23583537836495 41.0 39.0 41.0 34.0 41.0 21 38.18764039653592 40.0 38.0 41.0 34.0 41.0 22 38.13615312281498 40.0 38.0 41.0 34.0 41.0 23 38.06981533331485 40.0 38.0 41.0 34.0 41.0 24 38.02583054245371 40.0 38.0 41.0 33.0 41.0 25 37.96410336969287 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2892322066919917 1101 2 0.3858687276806094 1101 3 0.30186341510297154 1101 4 0.24142604540525525 1101 5 0.07775371225133654 1101 6 0.23422736583052028 1101 7 0.24731121840837744 1101 8 0.31443239389420796 1101 9 0.377422988571702 1101 10 0.4306771292541143 1101 11 0.3468622969247619 1101 12 0.3161640284603138 1101 13 0.37889609898250143 1101 14 0.45854653130957956 1101 15 0.40558479128056746 1101 16 0.38854187276993457 1101 17 0.32213867892848214 1101 18 0.2645033191502293 1101 19 0.2915079145506283 1101 20 0.31618869330898036 1101 21 0.3476361717085652 1101 22 0.30555525057948785 1101 23 0.34701517042565655 1101 24 0.3701798044258595 1101 25 0.37225934703917574 1102 1 0.5004476190207114 1102 2 0.5091643864766553 1102 3 0.459419300540425 1102 4 0.3431904734194262 1102 5 0.29935446250708253 1102 6 0.3738351452775106 1102 7 0.3834539175267082 1102 8 0.37578877626715723 1102 9 0.5723817373796223 1102 10 0.47633914379378695 1102 11 0.559141495846518 1102 12 0.5552696237876518 1102 13 0.5768929082169052 1102 14 0.7156709404683212 1102 15 0.7884869619320583 1102 16 0.7465978626846663 1102 17 0.73968808776538 1102 18 0.7722747446658573 1102 19 0.779339332797413 1102 20 0.7592275855924129 1102 21 0.761552544336908 1102 22 0.7763423159961249 1102 23 0.7098137352743592 1102 24 0.6878247777816782 1102 25 0.7303226652175283 1103 1 0.34423425639185723 1103 2 0.3454544005971698 1103 3 0.28071530126130995 1103 4 0.22725154537079106 1103 5 0.16872099487066805 1103 6 0.1127495814682149 1103 7 0.2730389584071844 1103 8 0.2619177896619931 1103 9 0.37971347748160156 1103 10 0.3925670008017548 1103 11 0.3962308097888254 1103 12 0.38840653527703495 1103 13 0.4294183524667545 1103 14 0.5549675796320983 1103 15 0.542606116848404 1103 16 0.6144066327426501 1103 17 0.5572356809986516 1103 18 0.5734045776833199 1103 19 0.6075592754142392 1103 20 0.6196879355089635 1103 21 0.5679189511208875 1103 22 0.5296213337527007 1103 23 0.5910758199150123 1103 24 0.6790161831516954 1103 25 0.7495298265144683 1104 1 0.405421769386777 1104 2 0.38769160616939047 1104 3 0.4056678594868046 1104 4 0.24887344350381113 1104 5 0.17973243700160424 1104 6 0.165075796060151 1104 7 0.20946698057198176 1104 8 0.2546274328008451 1104 9 0.31243691586340105 1104 10 0.39474024661119955 1104 11 0.3942027770630716 1104 12 0.39959784361391826 1104 13 0.40583772785247163 1104 14 0.5153491600124909 1104 15 0.4486796540741125 1104 16 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0.7236109722044475 1107 16 0.7655897656498922 1107 17 0.6554611953909273 1107 18 0.718585894246516 1107 19 0.7132406850994499 1107 20 0.6983518780313034 1107 21 0.7469048616293321 1107 22 0.6939731468811559 1107 23 0.8100857971679574 1107 24 0.7369354766585445 1107 25 0.7206216663862364 1108 1 0.1230391780083977 1108 2 0.11453559222632492 1108 3 0.12828159888127288 1108 4 0.07816830047042345 1108 5 0.009060862078911214 1108 6 0.05439940860039627 1108 7 0.04326927038243156 1108 8 0.03414377282652481 1108 9 0.08561287125835548 1108 10 -0.00589382508762526 1108 11 0.03925656580599224 1108 12 0.07995996708606867 1108 13 0.1439851935598213 1108 14 0.11022327627381401 1108 15 0.15730147374489434 1108 16 0.28994980090624267 1108 17 0.2935492392295558 1108 18 0.27054487235145785 1108 19 0.11029544565007399 1108 20 0.19996846792286505 1108 21 0.1422429640937679 1108 22 0.0027185680031607262 1108 23 -0.019102848562326358 1108 24 0.04030585431658551 1108 25 0.12756060389785517 1109 1 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-0.7002001773725794 2315 9 -0.7897784222593174 2315 10 -0.7744188973413983 2315 11 -0.7503360830467898 2315 12 -0.7579267810326655 2315 13 -0.8177155807426288 2315 14 -0.9596643870408386 2315 15 -1.0166006247559878 2315 16 -1.1374995158104895 2315 17 -1.006327270482302 2315 18 -0.9701045331048022 2315 19 -0.932447567779839 2315 20 -0.8901218951641567 2315 21 -0.9938585125631718 2315 22 -0.905015810558794 2315 23 -0.95698795644536 2315 24 -1.077322398215216 2315 25 -1.1215546418428133 2316 1 -1.1729124312787782 2316 2 -1.109718369534665 2316 3 -0.9685293260999437 2316 4 -0.781033821121909 2316 5 -0.7746126313899353 2316 6 -0.8674047315332558 2316 7 -0.8959271873371506 2316 8 -0.9355019008006735 2316 9 -1.1242483638592233 2316 10 -1.058471968831995 2316 11 -1.2157470134469293 2316 12 -1.2387336645970066 2316 13 -1.0673244590166462 2316 14 -1.3670504343101584 2316 15 -1.4086261034155427 2316 16 -1.4408372807439918 2316 17 -1.5456144742256797 2316 18 -1.7111414474292204 2316 19 -1.761056469643556 2316 20 -1.721452180826553 2316 21 -1.807791058475189 2316 22 -1.8104272216547628 2316 23 -2.0823858101384616 2316 24 -1.9850065436648734 2316 25 -1.8399483344013277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14662.0 3 1.0 4 324.0 5 2342.0 6 3069.0 7 2476.0 8 2018.0 9 1731.0 10 1762.0 11 1612.0 12 1437.0 13 1406.0 14 1468.0 15 1485.0 16 1705.0 17 1706.0 18 1816.0 19 2209.0 20 2634.0 21 3712.0 22 4665.0 23 5897.0 24 7430.0 25 8889.0 26 9995.0 27 11626.0 28 13854.0 29 17015.0 30 21926.0 31 29236.0 32 38112.0 33 51370.0 34 69429.0 35 96506.0 36 144601.0 37 269089.0 38 1423366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98754736075629 19.730460216964715 13.244526956622924 25.037465465656066 2 14.442913551279519 20.66358131308975 42.27481532103798 22.618689814592752 3 18.450123449945238 25.53730758111592 30.33498035933593 25.677588609602914 4 12.190672236330217 17.6025687454924 38.962988256798866 31.24377076137852 5 12.46536249007682 38.53951077077876 36.32592608256261 12.669200656581811 6 30.752791920849464 38.094208923846615 18.49866825012336 12.654330905180553 7 27.34769691234402 31.716014877087968 23.076564184951252 17.85972402561676 8 24.124673916248796 38.43032623999852 20.912449126093346 16.532550717659337 9 26.371281735245244 14.825341975811332 20.915251625967052 37.888124662976374 10 15.09253157932036 28.972686644490604 35.15526545028455 20.779516325904485 11 33.40772709024447 21.922915742247202 26.367656587853304 18.301700579655027 12 23.816329917346348 24.587491069734643 32.63690102294732 18.959277989971692 13 29.259892411687705 22.517401014263243 26.13180316497315 22.090903409075903 14 19.991057837572132 23.43137000353374 28.181321706175154 28.396250452718974 15 21.9681173630285 33.217739997729204 23.440612163528776 21.37353047571352 16 20.74714907117916 28.23434931936343 29.829021278281015 21.189480331176387 17 20.665688623070682 29.67414779077729 28.773447084438548 20.886716501713483 18 20.515671684480566 27.060552590239244 32.32538491663623 20.09839080864396 19 23.28028056218043 26.635585985971893 28.418537521231375 21.665595930616306 20 23.47502685272824 27.5146188408686 29.22958521610451 19.78076909029865 21 22.083730459416575 26.79379981677197 27.986542156088813 23.135927567722646 22 22.890687742443678 29.125233505028746 28.080451146017378 19.903627606510195 23 21.022315743525162 28.73681116412021 29.269424958395046 20.971448133959587 24 22.88560891778995 26.7758112912147 29.94894351400456 20.38963627699079 25 21.813523918399387 28.04854920462681 29.453999659418077 20.683927217555723 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 285.0 1 285.0 2 538.5 3 792.0 4 792.0 5 792.0 6 2021.0 7 3250.0 8 3250.0 9 3250.0 10 4379.5 11 5509.0 12 5509.0 13 5509.0 14 8331.0 15 11153.0 16 11153.0 17 11153.0 18 20459.0 19 29765.0 20 29765.0 21 29765.0 22 48021.5 23 66278.0 24 66278.0 25 66278.0 26 97049.5 27 127821.0 28 127821.0 29 127821.0 30 164928.5 31 202036.0 32 202036.0 33 202036.0 34 224270.5 35 246505.0 36 246505.0 37 246505.0 38 256921.0 39 267337.0 40 267337.0 41 267337.0 42 275408.5 43 283480.0 44 283480.0 45 283480.0 46 288318.5 47 293157.0 48 293157.0 49 293157.0 50 276130.5 51 259104.0 52 259104.0 53 259104.0 54 223108.0 55 187112.0 56 187112.0 57 187112.0 58 159036.5 59 130961.0 60 130961.0 61 130961.0 62 106756.0 63 82551.0 64 82551.0 65 82551.0 66 62924.0 67 43297.0 68 43297.0 69 43297.0 70 31603.0 71 19909.0 72 19909.0 73 19909.0 74 13919.5 75 7930.0 76 7930.0 77 7930.0 78 5483.5 79 3037.0 80 3037.0 81 3037.0 82 2004.5 83 972.0 84 972.0 85 972.0 86 610.5 87 249.0 88 249.0 89 249.0 90 161.0 91 73.0 92 73.0 93 73.0 94 43.0 95 13.0 96 13.0 97 13.0 98 9.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16831083248517875 2 0.00545635117076135 3 0.0 4 0.0014080906247126064 5 0.11629948503485683 6 0.033354146672879864 7 0.027765787006051708 8 0.02384953495606977 9 0.03766642421106223 10 0.03353015800096894 11 0.03894250633970802 12 0.03573029960208239 13 0.02582966239707188 14 0.008800566404453791 15 0.010868699509500432 16 0.00334421523369244 17 7.480481443785722E-4 18 4.840311522449585E-4 19 4.400283202226895E-5 20 0.0 21 1.3200849606680685E-4 22 0.0064244134752512675 23 6.600424803340343E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2272581.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.26616853301245 #Duplication Level Percentage of deduplicated Percentage of total 1 83.6699772504766 56.28158790883877 2 11.312788133616117 15.219358263481702 3 2.6573877872915053 5.362568842725583 4 0.892696753151452 2.4019316098543437 5 0.43308682737764864 1.4566045759906294 6 0.23371347971969536 0.9432606189157091 7 0.15416538616178183 0.7259080393260754 8 0.10739358761005371 0.5779164130834169 9 0.07040687724807027 0.4262400783766633 >10 0.4050973850078003 4.879737374047962 >50 0.03214905537341337 1.4757633839048008 >100 0.025005463168550707 3.5688372828146235 >500 0.0031312410879964017 1.411768604992293 >1k 0.0025441333839961496 3.0791413539578665 >5k 4.566393253326423E-4 2.1893756496896692 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8993 0.3957174683762647 No Hit CTGTAGGACGTGGAATATGGCAAGA 7631 0.3357856111619344 No Hit GTCCTACAGTGGACATTTCTAAATT 7469 0.3286571523743268 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7182 0.3160283395839356 No Hit CTTTAGGACGTGAAATATGGCGAGG 6720 0.29569903118964735 No Hit GGTATCAACGCAGAGTACTTTTTTT 6132 0.26982536596055323 No Hit TATCAACGCAGAGTACTTTTTTTTT 5767 0.25376433227242506 No Hit GTCCTACAGTGTGCATTTCTCATTT 3957 0.17411920631211825 No Hit CTGAAGGACCTGGAATATGGCGAGA 3793 0.16690274186046614 No Hit CTGTAGGACCTGGAATATGGCGAGA 2984 0.13130445075445055 No Hit ATTTAGAAATGTCCACTGTAGGACG 2960 0.1302483827859161 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2851 0.12545207409548878 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2825 0.1243080004629098 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2820 0.12408798630279844 No Hit GAATATGGCAAGAAAACTGAAAATC 2683 0.1180595983157476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.4002832022268956E-5 0.0 4 0.0 0.0 0.0 1.7601132808907582E-4 0.0 5 0.0 0.0 0.0 1.7601132808907582E-4 0.0 6 0.0 0.0 0.0 1.7601132808907582E-4 4.4002832022268956E-5 7 0.0 0.0 0.0 1.7601132808907582E-4 4.4002832022268956E-5 8 0.0 0.0 0.0 1.7601132808907582E-4 4.4002832022268956E-5 9 0.0 0.0 0.0 2.640169921336137E-4 4.4002832022268956E-5 10 0.0 0.0 0.0 2.640169921336137E-4 8.800566404453791E-5 11 0.0 0.0 0.0 2.640169921336137E-4 8.800566404453791E-5 12 0.0 0.0 0.0 2.640169921336137E-4 1.7601132808907582E-4 13 0.0 4.4002832022268956E-5 0.0 2.640169921336137E-4 2.2001416011134477E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1565 0.0 13.621133 1 TAGGACC 1160 0.0 12.457875 4 ACCTTTT 995 0.0 11.641833 15 CACCTTT 1015 0.0 11.505982 14 GGCGAGG 1660 0.0 11.267647 19 CGGTTTC 85 5.3442753E-5 11.172534 13 GTCTAAC 95 1.3348432E-5 11.019165 1 TAGAAAT 1065 0.0 10.98029 4 AATGTCC 1085 0.0 10.943496 8 GAAATGT 1070 0.0 10.830584 6 ATTTAGA 1030 0.0 10.810062 1 TGGTATC 465 0.0 10.631379 2 AGGACCT 2430 0.0 10.563929 5 GTGGTAT 505 0.0 10.553007 1 GGACCTG 2400 0.0 10.5280075 6 CCACCTT 1075 0.0 10.512576 13 CCCGTAC 100 2.4149127E-5 10.444022 16 ATAGTAC 210 0.0 10.412557 3 TAGGACA 1115 0.0 10.402634 4 TGTCCAC 1125 0.0 10.385066 10 >>END_MODULE