FastQCFastQC Report
Thu 26 May 2016
SRR1512424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482966
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68510.2759200085703952No Hit
GTCCTACAGTGGACATTTCTAAATT59430.23935084088948458No Hit
CTGTAGGACGTGGAATATGGCAAGA54590.21985802463666435No Hit
GTCCTAAAGTGTGTATTTCTCATTT50720.20427182651715728No Hit
GGTATCAACGCAGAGTACTTTTTTT42550.1713676304870868No Hit
CTTTAGGACGTGAAATATGGCGAGG41420.16681662173384573No Hit
TATCAACGCAGAGTACTTTTTTTTT41050.16532646842526239No Hit
GTCCTACAGTGTGCATTTCTCATTT32560.131133491155336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1500.013.89693519
GGTATCA15350.013.6964181
TCCAACG2050.013.40093418
TAGGACC10850.013.3740374
GTCCTAG3800.012.9873991
GGCGAGG13250.011.79928419
ACCGTGC1157.575545E-811.5089248
ATCCCGT1701.2732926E-1111.15156110
GTCCTAA16400.011.1343191
ATTGCGA600.005936232411.06848917
GTAGGAC33200.010.9411133
AGGACGT31700.010.9202965
GTACTAG1457.95626E-910.8915011
TAAGACG700.001512796710.8390884
TGTAGGA35050.010.8234072
AAATGTC7050.010.7626527
GACGTGG15450.010.7430597
GGACGTG31700.010.6813796
AGGACCT21400.010.6808075
TCCCGTT2050.010.63607411
Produced by FastQC (version 0.11.5)