FastQCFastQC Report
Thu 26 May 2016
SRR1512424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482966
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86580.3486958742085071No Hit
CTGTAGGACGTGGAATATGGCAAGA63510.2557828016976471No Hit
GTCCTACAGTGGACATTTCTAAATT62920.2534066112866628No Hit
GGTATCAACGCAGAGTACTTTTTTT57010.22960443276307446No Hit
TATCAACGCAGAGTACTTTTTTTTT55090.2218717453239392No Hit
GTCCTAAAGTGTGTATTTCTCATTT53640.21603195533084224No Hit
CTTTAGGACGTGAAATATGGCGAGG49310.1985931341790423No Hit
GTCCTACAGTGTGCATTTCTCATTT36390.1465585916198611No Hit
CTGAAGGACCTGGAATATGGCGAGA27650.11135875400629731No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27400.11035189366265989No Hit
CTGTAGGACCTGGAATATGGCGAGA27240.10970750304273195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.013.5073751
TAGGACC10950.012.8499944
GGCGAGG12800.011.86899719
TCCAACG2400.011.47336318
GTGCGCC1001.9351828E-611.39790811
TGTAGGA33450.011.3123032
GTAGGAC33600.011.2615753
CCGGGGG1950.011.19946316
AGGACCT20050.011.0962015
GGACCTG19550.010.8829156
GACGTGG16500.010.8798237
CTGTAGG32350.010.8536381
TTGCGAG700.001498253310.85165318
CGTTTCC1605.493348E-1010.68252714
AGGACGT33650.010.651955
TAGGACG34950.010.6089574
GGACGTG33400.010.5788976
GTCCTAC30500.010.5760411
CGCCCTA3150.010.5502216
TCCTACA32300.010.537672
Produced by FastQC (version 0.11.5)