FastQCFastQC Report
Thu 26 May 2016
SRR1512423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944368
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74900.38521514445825067No Hit
GGTATCAACGCAGAGTACTTTTTTT48630.2501069756342421No Hit
TATCAACGCAGAGTACTTTTTTTTT45220.2325691432897476No Hit
GTCCTACAGTGGACATTTCTAAATT40970.21071114110086156No Hit
CTGTAGGACGTGGAATATGGCAAGA38620.19862495165524222No Hit
GTCCTAAAGTGTGTATTTCTCATTT38320.1970820338536738No Hit
CTTTAGGACGTGAAATATGGCGAGG31920.16416645408688066No Hit
GTCCTACAGTGTGCATTTCTCATTT22940.1179817812265991No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21460.11037005340552818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12050.014.82440951
CTGTCGA400.005441674514.1852419
TGTCGAG1251.07320375E-1012.88521918
ACCGTGC854.171143E-612.23288258
TAGGACC8850.011.7886144
GGCGAGG11950.011.41806219
TTAGGAC17250.011.1614423
GACGTGA13900.011.1226597
AATCCCG1053.5763842E-610.82903519
GTCCTAT2300.010.7274881
AAGACGT909.673458E-510.5385795
CGTGCGC909.744734E-510.5309510
GGACCTG15350.010.50425156
GTATCAA27050.010.4348131
GTCCTAA12500.010.4219691
GGACGTG24850.010.3434996
AGGACCT16200.010.3043895
AGGACGT25900.010.2171335
TGGCGAG25350.010.20322818
GTCCTAC21300.010.1936321
Produced by FastQC (version 0.11.5)