FastQCFastQC Report
Thu 26 May 2016
SRR1512423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944368
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91770.4719785554997819No Hit
GGTATCAACGCAGAGTACTTTTTTT63770.327972894020062No Hit
TATCAACGCAGAGTACTTTTTTTTT59980.3084806991269143No Hit
CTGTAGGACGTGGAATATGGCAAGA44930.23107765608156478No Hit
GTCCTACAGTGGACATTTCTAAATT41850.21523703331879562No Hit
GTCCTAAAGTGTGTATTTCTCATTT40800.20983682101330614No Hit
CTTTAGGACGTGAAATATGGCGAGG37720.19399619825053693No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28770.14796581717041218No Hit
GTCCTACAGTGTGCATTTCTCATTT23640.12158192276359207No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21960.11294158307480888No Hit
CTGAAGGACCTGGAATATGGCGAGA21330.1097014556915152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG350.00215917716.298432
GGTATCA15750.013.5932271
GGACCGT550.00307169312.0894526
TAGGACC7150.011.8344074
AGGACCG752.0593418E-411.4089015
CGATAAG752.0771436E-411.39833412
TCGAACT1201.2860437E-711.07686719
GGCGAGG11600.010.96773519
GTCCTAT2550.010.8212751
AGCGTAC803.7160658E-410.7046661
CTAATAC1803.45608E-1110.5637973
TGGCGAG25350.010.26225418
TAGAACG750.002630991210.1412454
GTGCTAC1606.01176E-910.1099621
GTGGTAT5000.010.0861731
GTATAAG1701.5170372E-910.074981
TGCGATA856.601836E-410.05761110
AATGTCC6250.010.0307918
AAATGTC6450.010.0142977
CCACCTT6850.09.98188613
Produced by FastQC (version 0.11.5)