FastQCFastQC Report
Thu 26 May 2016
SRR1512422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763234
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83390.3017840689568817No Hit
GTCCTACAGTGGACATTTCTAAATT66220.2396467327776077No Hit
CTGTAGGACGTGGAATATGGCAAGA63490.22976700489354143No Hit
GTCCTAAAGTGTGTATTTCTCATTT58020.20997135964598004No Hit
CTTTAGGACGTGAAATATGGCGAGG50210.18170737621207614No Hit
GGTATCAACGCAGAGTACTTTTTTT49490.17910173369320154No Hit
TATCAACGCAGAGTACTTTTTTTTT47690.17258762739601496No Hit
GTCCTACAGTGTGCATTTCTCATTT31820.11515492354248681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.013.745961
TCCAACG1457.2759576E-1212.41392618
TAGGACC11150.011.9929494
GGCGAGG15100.011.54715419
TCGCGCG600.00607233311.0348979
AGCGACC600.006095331611.0292928
AATCCCG2150.011.01883519
GGACGTG36750.010.99364956
TATCGCG700.001513215510.838817
AGGACGT37550.010.8352015
TGTAGGA39900.010.7435362
TTAGGAC21300.010.6414313
GACGTGG19950.010.5535777
GTATCAA36400.010.4884421
GTCTAGG2850.010.3843131
GTACTAG1904.9112714E-1110.3843131
CTGTAGG37650.010.37603951
GTAGGAC38600.010.3682843
GACGTGA19350.010.3416817
AGGACCT23650.010.3060355
Produced by FastQC (version 0.11.5)