FastQCFastQC Report
Thu 26 May 2016
SRR1512422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763234
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT102350.37039932195391345No Hit
CTGTAGGACGTGGAATATGGCAAGA74980.2713487167572489No Hit
GTCCTACAGTGGACATTTCTAAATT69100.2500693028531062No Hit
GGTATCAACGCAGAGTACTTTTTTT67970.24597989167765016No Hit
TATCAACGCAGAGTACTTTTTTTTT67030.24257808061134162No Hit
GTCCTAAAGTGTGTATTTCTCATTT62670.22679946758037864No Hit
CTTTAGGACGTGAAATATGGCGAGG59680.2159788132311632No Hit
GTCCTACAGTGTGCATTTCTCATTT34680.12550511465912767No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32870.1189548188825123No Hit
CTGAAGGACCTGGAATATGGCGAGA32680.11826721877336484No Hit
CTGTAGGACCTGGAATATGGCGAGA28180.10198195303039845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12100.013.5932274
GGTATCA17500.013.4843421
ACCGTGC905.4160046E-712.665528
ACTACGT701.0924152E-412.2131818
TGTAGGA37650.011.6153172
CGTGCGC1157.093695E-811.56375110
AGGACCT23400.011.5391065
GTAGGAC37850.011.2529423
GGCGAGG15450.011.12340719
CTGTAGG37850.011.03607751
GGACCTG23700.010.9021646
GTGGTAT6700.010.65129851
GGACGTG36450.010.6329756
CGCCCTA1701.4006218E-1010.61188316
AGGACGT37300.010.60362155
TGGCGAG34750.010.41017218
GCGTGCG1201.5266105E-610.2907349
GACGTGG20200.010.2515487
TAGGACG39450.010.1219494
TGGTATC6200.010.1202332
Produced by FastQC (version 0.11.5)