FastQCFastQC Report
Thu 26 May 2016
SRR1512421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588616
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67370.2602549006882442No Hit
GTCCTACAGTGGACATTTCTAAATT66140.2555033268742834No Hit
CTGTAGGACGTGGAATATGGCAAGA56890.21976994656604149No Hit
GTCCTAAAGTGTGTATTTCTCATTT52830.20408588991182933No Hit
CTTTAGGACGTGAAATATGGCGAGG48260.18643166850548712No Hit
GGTATCAACGCAGAGTACTTTTTTT41990.16221023125871123No Hit
TATCAACGCAGAGTACTTTTTTTTT40720.15730413471909316No Hit
GTCCTACAGTGTGCATTTCTCATTT35720.13798879401193534No Hit
CTGTAGGACCTGGAATATGGCGAGA26690.10310528869480835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12800.013.3358724
GGCGAGG15150.011.82030419
GGTATCA12150.011.4521631
GTGTAGG5050.011.1385361
TCCAACG2050.011.09075318
TGTAGGA37750.010.97800352
AGGACCT22850.010.9568335
GGACCTG22250.010.9541556
GTATCAA28800.010.8277761
GTCCTAC33100.010.7031381
GTAGGAC36250.010.6997633
CCAACGT803.8654366E-410.65938119
ACTGCGC803.9423333E-410.6366758
GTCCTAT3650.010.5442681
TGGCGAG35900.010.47610118
CTGTAGG34250.010.4013881
TCCTACA37200.010.3755312
GGACGTG35100.010.375286
AGGACGT35100.010.3750785
TAGGACT4150.010.2830824
Produced by FastQC (version 0.11.5)