FastQCFastQC Report
Thu 26 May 2016
SRR1512421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588616
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87130.3365891271629319No Hit
GTCCTACAGTGGACATTTCTAAATT69430.26821282105959326No Hit
CTGTAGGACGTGGAATATGGCAAGA65220.2519493041841664No Hit
GGTATCAACGCAGAGTACTTTTTTT57960.22390342947737324No Hit
TATCAACGCAGAGTACTTTTTTTTT57720.22297629312342965No Hit
CTTTAGGACGTGAAATATGGCGAGG55540.21455480457510884No Hit
GTCCTAAAGTGTGTATTTCTCATTT55370.2138980829910655No Hit
GTCCTACAGTGTGCATTTCTCATTT40430.15618384495807797No Hit
CTGTAGGACCTGGAATATGGCGAGA31250.12072087941973626No Hit
CTGAAGGACCTGGAATATGGCGAGA29440.11372872608374512No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27430.1059639591194677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.014.2290891
TAGGACC13050.012.9682364
GGCGAGG15700.011.79330419
TGTAGGA40200.011.2349862
AGGACGT37850.010.7007735
GTAGGAC40700.010.6989833
GGACGTG37700.010.6821666
CTGTAGG40700.010.6617481
TAGAAAT9250.010.5868534
TTAGGAC22150.010.5592433
TGGTATC4600.010.5418632
GACGTGG19150.010.5146197
CGACTAA1553.6961865E-910.41400517
AATGTCC8600.010.3811978
GACGTGA19750.010.3394587
AAATGTC8850.010.3027797
TTTAGGA24050.010.2792942
GTCCTAG3900.010.2480841
GCGCCAC1303.861096E-710.22749213
TAGGACG39700.010.226074
Produced by FastQC (version 0.11.5)