FastQCFastQC Report
Thu 26 May 2016
SRR1512420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382138
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81870.3436828596831921No Hit
GTCCTACAGTGGACATTTCTAAATT58100.24389854827889906No Hit
CTGTAGGACGTGGAATATGGCAAGA54150.22731680532362106No Hit
GTCCTAAAGTGTGTATTTCTCATTT49770.20892996123650265No Hit
GGTATCAACGCAGAGTACTTTTTTT48860.2051098634923753No Hit
TATCAACGCAGAGTACTTTTTTTTT46730.196168316025352No Hit
CTTTAGGACGTGAAATATGGCGAGG40400.1695955482008179No Hit
GTCCTACAGTGTGCATTTCTCATTT29170.12245302329252125No Hit
GTACTGGTTCACTATCGGTCAGTCA27850.11691178260873215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.013.8469181
TAGGACC9350.013.5923194
GGACCGT802.03745E-613.0413156
AGGACCG1251.05501385E-1012.8990485
TCCAACG2500.012.506721518
CCAACGT1551.8189894E-1212.23017819
GGCGAGG13050.011.69364319
CGAATGG1251.8959327E-811.37406619
GTATTAG2900.011.2294211
TGTAGGA32000.011.1443982
GTAGGAC30650.010.8616383
AGGACCT19150.010.7970835
GACGTGG16550.010.7171837
GACCGTG803.827746E-410.6706187
GTCCTAC26950.010.6555441
GGACCTG19000.010.6327296
ACTGTTC7350.010.5451518
CCAACGA1355.8846126E-810.53154319
AGGACGT33150.010.4716795
GTATCAA33650.010.440141
Produced by FastQC (version 0.11.5)