FastQCFastQC Report
Thu 26 May 2016
SRR1512420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382138
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT103700.43532322644615884No Hit
TATCAACGCAGAGTACTTTTTTTTT66170.27777567882297327No Hit
GGTATCAACGCAGAGTACTTTTTTT65510.27500505848107876No Hit
CTGTAGGACGTGGAATATGGCAAGA64820.27210850085091626No Hit
GTCCTACAGTGGACATTTCTAAATT60510.25401551043642306No Hit
GTCCTAAAGTGTGTATTTCTCATTT51960.21812338328006187No Hit
CTTTAGGACGTGAAATATGGCGAGG48990.20565559174153641No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32160.13500477302322536No Hit
GTACTGGTTCACTATCGGTCAGTCA30400.12761645211150655No Hit
GTCCTACAGTGTGCATTTCTCATTT30340.12736457753497069No Hit
CTGAAGGACCTGGAATATGGCGAGA29210.1226209396768785No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23870.10020410236518623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.014.2366661
TCCAACG2350.014.14104318
CTACACG707.2123494E-613.5812474
TAGGACC9450.012.9776364
CGGTTTC1750.012.48136913
TTGCGAG701.09707085E-412.20747118
CCAACGA1900.011.99330619
GCCGGTT1750.011.94020511
CACACCG657.9748133E-411.7012595
TGCCGGT1950.011.69020210
GGCGAGG12600.011.227859519
ACCGTGC855.3328855E-511.1749278
CGTTTCC1701.2732926E-1111.17070414
ACCGTCC600.00588326311.0818018
TGTAGGA32050.011.0349622
CCGGTTT1900.010.99709512
CTGTAGG32250.010.5356231
GTAGGAC32500.010.530693
GTCCTAC27000.010.4692221
ACTGTTC8950.010.4007978
Produced by FastQC (version 0.11.5)