FastQCFastQC Report
Thu 26 May 2016
SRR1512419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836550
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76050.26810738396996353No Hit
GTATCAACGCAGAGTACTTTTTTTT70650.24907017327387143No Hit
GTCCTAAAGTGTGTATTTCTCATTT64310.22671907775290404No Hit
CTGTAGGACGTGGAATATGGCAAGA60740.21413336623715432No Hit
CTTTAGGACGTGAAATATGGCGAGG51820.1826867144947207No Hit
GTCCTACAGTGTGCATTTCTCATTT50420.17775114135128942No Hit
GGTATCAACGCAGAGTACTTTTTTT43230.15240344785038162No Hit
TATCAACGCAGAGTACTTTTTTTTT42370.14937159577655956No Hit
CTGTAGGACCTGGAATATGGCGAGA31420.11076836297615061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.013.7383981
TAGGACC12900.012.9381724
TATACCG604.154594E-412.6445715
TAGGCCG550.003109905412.0698185
TGCGATG658.1453886E-411.6719125
TGTAGGA39050.011.3898292
ATGTCGA1501.8007995E-1011.38556517
GGCGAGG16650.011.32571219
GTGTAGG5450.011.0598721
GTAGGAC38700.010.8802133
GTCCTAC39450.010.87074851
TAGGACA11600.010.7911054
CTGTAGG36650.010.59583951
AGGACCT22650.010.5929695
GTATCAA29650.010.4979041
TTAGGAC23450.010.4742353
GGACCTG22700.010.4443056
GACGTGA20350.010.4389477
TGGTATC4650.010.401182
GCCTTAT2400.010.2930841
Produced by FastQC (version 0.11.5)