FastQCFastQC Report
Thu 26 May 2016
SRR1512419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836550
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82730.29165711868290706No Hit
GTCCTACAGTGGACATTTCTAAATT75430.2659216301493011No Hit
CTGTAGGACGTGGAATATGGCAAGA74330.26204367982231935No Hit
CTTTAGGACGTGAAATATGGCGAGG63480.22379298796072694No Hit
GTCCTAAAGTGTGTATTTCTCATTT60930.21480319402090567No Hit
GGTATCAACGCAGAGTACTTTTTTT52910.18652941072782078No Hit
TATCAACGCAGAGTACTTTTTTTTT52490.1850487387847914No Hit
GTCCTACAGTGTGCATTTCTCATTT49910.17595318256332515No Hit
CTGTAGGACCTGGAATATGGCGAGA40060.14122790008989794No Hit
CTGAAGGACCTGGAATATGGCGAGA32680.11521037880523875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.013.3275311
TAGGACC13850.012.9741484
TACACCG1103.7765858E-812.1004685
GGCGAGG18150.011.71868619
TGTAGGA42000.011.1145362
TCGAACT1658.185452E-1110.93395319
CTGTAGG41750.010.7345541
TTAGGAC24600.010.6283153
GTAGGAC43100.010.6104713
GTACGAC909.4445284E-510.5639013
GTATCAA33900.010.5537721
AGGACGT39800.010.5347045
GGACGTG39250.010.5269526
GTCCTAC38000.010.4667611
GTAGAAC7050.010.384093
TAGAAAT11000.010.371834
CTAATAC2400.010.2998033
TAGGACG42450.010.2802064
GACGTGG20050.010.1851987
AGGACCT25600.010.1760075
Produced by FastQC (version 0.11.5)