FastQCFastQC Report
Thu 26 May 2016
SRR1512418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984655
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70670.3560820394476622No Hit
GTCCTACAGTGGACATTTCTAAATT50010.2519833421929756No Hit
GTCCTAAAGTGTGTATTTCTCATTT48080.242258730106744No Hit
GGTATCAACGCAGAGTACTTTTTTT45860.23107290687802165No Hit
CTGTAGGACGTGGAATATGGCAAGA45170.227596232090716No Hit
TATCAACGCAGAGTACTTTTTTTTT41260.20789507496265092No Hit
CTTTAGGACGTGAAATATGGCGAGG40230.2027052560772527No Hit
GTCCTACAGTGTGCATTTCTCATTT22130.11150552614938111No Hit
CTGAAGGACCTGGAATATGGCGAGA21460.10812962454431625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.014.0727711
GGCGAGT500.001528670513.26638319
GTGTAGG3950.013.2499041
TAGGACC8600.012.2411784
TATGTCG1157.199196E-811.55090716
TGCACCG1001.9693998E-611.3809875
AATGTCC7100.011.3155468
TAGAAAT8100.011.1233934
AGCGTGC600.00610328211.027118
CCTAGAC1605.6024874E-1010.6696753
TGTCCAC7650.010.65517310
TGTAGGA28950.010.6468832
CACCTTT7800.010.58384814
GGCGAGG12750.010.5536519
GTCCTAC22850.010.5447811
TGTCGAG1451.506487E-810.45414518
AGGACCT16350.010.3832665
GTATTAG2001.0913936E-1110.3686731
GTATCAA27500.010.34174251
CCACCTT8050.010.25203613
Produced by FastQC (version 0.11.5)