FastQCFastQC Report
Thu 26 May 2016
SRR1512418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984655
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88650.4466771302820893No Hit
GGTATCAACGCAGAGTACTTTTTTT61230.3085170974300319No Hit
TATCAACGCAGAGTACTTTTTTTTT59100.2977847535213929No Hit
CTGTAGGACGTGGAATATGGCAAGA54060.2723899115967259No Hit
GTCCTACAGTGGACATTTCTAAATT52960.26684738657348506No Hit
GTCCTAAAGTGTGTATTTCTCATTT50350.2536964862910682No Hit
CTTTAGGACGTGAAATATGGCGAGG48630.24502999261836442No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27120.1366484351184463No Hit
CTGAAGGACCTGGAATATGGCGAGA26000.13100513691296473No Hit
GTCCTACAGTGTGCATTTCTCATTT23840.12012163323096457No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22770.11473026798108486No Hit
ATTTAGAAATGTCCACTGTAGGACG22050.11110243342041816No Hit
TTTCTAAATTTTCCACCTTTTTCAG21420.10792807817983478No Hit
GTCCTTCAGTGTGCATTTCTCATTT20640.10399792407244585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1006.657501E-1014.24174319
GGTATCA14550.014.1945081
TAGGACC8450.013.2766484
TCGAACT1001.4541365E-712.34284419
TCCAACG1405.2750693E-1112.20720918
GTCGAGG855.3558775E-511.16999419
GGCGAGG13550.011.14114219
TAAGACG600.00584253111.0920014
CTATACT2900.010.81884
TGTCGAG1158.885381E-710.73290718
GGACCTG17300.010.5963966
TGTAGGA28750.010.5827182
AGGACCT18300.010.5464945
TTAGGAC18050.010.4818763
CCACCTT7450.010.45347713
CTGTAGG28750.010.3947821
CACCTTT7900.010.33630114
AGGACGT30850.010.3241135
GGACGTG29800.010.2951626
GTATCAA31300.010.1864791
Produced by FastQC (version 0.11.5)