FastQCFastQC Report
Thu 26 May 2016
SRR1512417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263465
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80490.3556052335688867No Hit
GGTATCAACGCAGAGTACTTTTTTT52460.23176854954682313No Hit
TATCAACGCAGAGTACTTTTTTTTT47700.21073884508927684No Hit
GTCCTACAGTGGACATTTCTAAATT46460.20526051871798326No Hit
GTCCTAAAGTGTGTATTTCTCATTT43250.19107872222455394No Hit
CTGTAGGACGTGGAATATGGCAAGA41420.18299377282175777No Hit
CTTTAGGACGTGAAATATGGCGAGG38160.1685910760714215No Hit
GTCCTACAGTGTGCATTTCTCATTT25950.11464723333473237No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23640.10444164146562902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.014.3477861
TAGGACC8250.012.1856774
AGTGCGG550.003125463112.061604510
GTGCGGA600.00441169911.5180841
AATCCCG1251.9012077E-811.37186519
GGCGAAA752.1227579E-411.37186519
GTGTAGG4900.011.2830211
AGGACCT17650.010.9618325
CGTGCGC1303.38714E-810.93497210
GTATCAA32600.010.8114531
GGACCTG17200.010.6972226
GGCGAGG13200.010.55343419
TAGAACG909.681145E-510.5379284
AGGCGTG2001.8189894E-1210.43254857
TGTAGGA28500.010.282572
GTCCTAA15100.010.2649341
GTATAAG2600.010.252361
ATTACAC2500.010.2428663
GTCCTAT2800.010.2252381
GCGCCAC2053.6379788E-1210.18013213
Produced by FastQC (version 0.11.5)