FastQCFastQC Report
Thu 26 May 2016
SRR1512417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263465
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98370.43459916543882937No Hit
GGTATCAACGCAGAGTACTTTTTTT68410.3022357315001557No Hit
TATCAACGCAGAGTACTTTTTTTTT65320.2885840956233032No Hit
GTCCTACAGTGGACATTTCTAAATT50160.22160713772910118No Hit
CTGTAGGACGTGGAATATGGCAAGA48450.21405234894288183No Hit
GTCCTAAAGTGTGTATTTCTCATTT46330.2046861780500251No Hit
CTTTAGGACGTGAAATATGGCGAGG43890.1939062455129635No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31760.14031584318732562No Hit
GTCCTACAGTGTGCATTTCTCATTT27420.12114170088779813No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25240.111510449686653No Hit
CTGAAGGACCTGGAATATGGCGAGA22750.10050961689268445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.013.9516221
TAGGACC8200.012.7523144
GGACCGT600.00588011511.082566
GTGGTAT5850.010.8974061
CGTGCGC700.001493001810.85614510
GGCGAGG11950.010.7264919
CACCTTT6650.010.5660214
AGGCGTG1355.696529E-810.5548187
GCGTGCG909.527424E-510.5545859
TGTAGGA27950.010.5096162
AAATGTC7500.010.3859427
GCGCCAC1106.0674156E-610.36085213
GTCCTAA13400.010.2959731
GGTCTAC2500.010.2760911
AATGTCC7900.010.2205798
GTATTAG2053.6379788E-1210.2111111
TGTACCG750.002633911810.1400215
GTAGGAC28050.010.0993543
AGGACGT28800.010.0343975
GCCGTGC951.6460997E-49.9990818
Produced by FastQC (version 0.11.5)