FastQCFastQC Report
Thu 26 May 2016
SRR1512416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838249
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60700.33020553798750873No Hit
GTCCTACAGTGGACATTTCTAAATT59590.32416718300948344No Hit
GTCCTAAAGTGTGTATTTCTCATTT57030.3102408868439477No Hit
CTGTAGGACGTGGAATATGGCAAGA56940.30975129049437805No Hit
CTTTAGGACGTGAAATATGGCGAGG46190.2512717265180071No Hit
GGTATCAACGCAGAGTACTTTTTTT39350.21406240395071613No Hit
TATCAACGCAGAGTACTTTTTTTTT37010.20133289886190608No Hit
CTGAAGGACCTGGAATATGGCGAGA25250.13735897585147605No Hit
GTCCTACAGTGTGCATTTCTCATTT23570.12821984399284317No Hit
GTCCTTCAGTGTGCATTTCTCATTT23350.12702305291611746No Hit
ATTTAGAAATGTCCACTGTAGGACG22050.11995110564455631No Hit
TTTCTAAATTTTCCACCTTTTTCAG19670.10700400217815975No Hit
GAATATGGCAAGAAAACTGAAAATC19610.10667760461178001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA400.00533518914.2275077
CGACACT400.005336157514.2271186
GGTATCA10500.013.8256781
TAGGACC8450.012.7956374
TCCAACG951.0763524E-611.96599818
GTTTAGG2500.011.4555611
TAGAACG752.1059178E-411.3813834
GTATTAG1404.5547495E-911.2862671
CAAGTCG2400.011.0655366
TTATACG600.00594824611.0652334
CCACCTT7600.010.98233613
GCCGGTT951.3945815E-510.97542911
AAGTCGT2600.010.9442367
GGACCTG19450.010.92330556
TATGTCG1408.5055945E-910.84445716
AGGACCT20300.010.8394125
GTATCAA23800.010.746851
TTAGGAC19450.010.679193
GGCGAGG15100.010.66680719
ATAATTC1252.2913082E-710.6226243
Produced by FastQC (version 0.11.5)