FastQCFastQC Report
Thu 26 May 2016
SRR1512416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838249
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76410.41566730078460534No Hit
CTGTAGGACGTGGAATATGGCAAGA64530.35104058264141585No Hit
GTCCTACAGTGGACATTTCTAAATT63450.3451654264465804No Hit
GTCCTAAAGTGTGTATTTCTCATTT61050.3321095237913906No Hit
CTTTAGGACGTGAAATATGGCGAGG55920.30420253186592244No Hit
GGTATCAACGCAGAGTACTTTTTTT54960.2989801708038465No Hit
TATCAACGCAGAGTACTTTTTTTTT51410.2796683147930449No Hit
CTGAAGGACCTGGAATATGGCGAGA30890.16804034709117208No Hit
GTCCTACAGTGTGCATTTCTCATTT25930.1410581482704465No Hit
ATTTAGAAATGTCCACTGTAGGACG25800.14035095354329039No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25440.1383925681450119No Hit
TTTCTAAATTTTCCACCTTTTTCAG24900.1354549900475942No Hit
GTCCTTCAGTGTGCATTTCTCATTT24250.13191901641181364No Hit
GAATATGGCAAGAAAACTGAAAATC21670.11788392105748459No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19720.10727600015014288No Hit
CTGTAGGACCTGGAATATGGCGAGA19660.10694960258376314No Hit
GGAATATGGCGAGAAAACTGAAAAT18900.10281523340961969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG250.006037280418.9973876
GGTATCA12650.013.839631
GCCGGTT604.098349E-412.6645811
TAGGACC7600.012.6328894
GCGCCAC1001.4491525E-712.3459513
TCCAACG1001.4517173E-712.34427118
GGCGAGG13950.011.70782819
CACCTTT7800.011.44371914
ACCTTTT8350.011.14483215
AATGTCC8600.010.8240948
CCACCTT8350.010.80483913
ACGAAAT2200.010.79338312
CCGGGGG1158.870793E-710.73414816
TTCCACC8700.010.5902111
AAATGTC8750.010.4214257
AGGACCT17950.010.3267845
TAGAAAT9850.010.3262454
GACGTGG16850.010.2033457
GTCCTAT2151.8189894E-1210.1785651
GGCGTGC1409.7079464E-810.1771728
Produced by FastQC (version 0.11.5)