FastQCFastQC Report
Thu 26 May 2016
SRR1512415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250511
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT108410.3335167916675255No Hit
CTGTAGGACGTGGAATATGGCAAGA96610.2972148071487837No Hit
GTCCTAAAGTGTGTATTTCTCATTT85420.26278945064329884No Hit
GTCCTACAGTGTGCATTTCTCATTT78780.2423618932530916No Hit
GTATCAACGCAGAGTACTTTTTTTT74200.22827180095683416No Hit
CTTTAGGACGTGAAATATGGCGAGG69160.21276654655221902No Hit
CTGTAGGACCTGGAATATGGCGAGA50830.1563754129735294No Hit
GGTATCAACGCAGAGTACTTTTTTT46350.1425929646138715No Hit
TATCAACGCAGAGTACTTTTTTTTT41830.12868745867957376No Hit
CTGAAGGACCTGGAATATGGCGAGA38300.11782762771761117No Hit
GTCCTTCAGTGTGCATTTCTCATTT33000.10152249907783731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC23650.014.5565864
AGACCGT801.3157478E-714.2268546
GGTATCA16200.013.901361
TATTGCG1051.3969839E-913.55505116
CGATAAA602.946477E-413.169711
AGGACCT40450.012.2156535
GGACCTG40750.011.8004346
TGTAGGA67550.011.7372122
GTGTAGG7850.011.4500981
GTAGGAC66950.011.3749363
GGCGAGG20550.011.29627419
CTGTAGG61000.011.140281
GTCCTAC58250.011.0727311
GTCCTAG5800.011.0693681
TAGAAAT12950.011.05837254
GACGTGG29900.011.0071767
AATGTCC12750.010.8245768
CTAGGAC5700.010.81493
TGGCGAG55700.010.69750318
TCCTACA66550.010.688022