FastQCFastQC Report
Thu 26 May 2016
SRR1512415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250511
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA114460.35212924983179567No Hit
GTCCTACAGTGGACATTTCTAAATT112660.34659165897300453No Hit
GTATCAACGCAGAGTACTTTTTTTT91340.28100197168998964No Hit
GTCCTAAAGTGTGTATTTCTCATTT86440.26592741879661386No Hit
GTCCTACAGTGTGCATTTCTCATTT84610.26029753475684286No Hit
CTTTAGGACGTGAAATATGGCGAGG84310.2593746029470443No Hit
CTGTAGGACCTGGAATATGGCGAGA61030.1877550945066791No Hit
GGTATCAACGCAGAGTACTTTTTTT57490.17686449915105654No Hit
TATCAACGCAGAGTACTTTTTTTTT56760.17461869841388014No Hit
CTGAAGGACCTGGAATATGGCGAGA48640.1496380107620002No Hit
GAATATGGCAAGAAAACTGAAAATC34810.10709085433028838No Hit
GTCCTTCAGTGTGCATTTCTCATTT33570.10327606951645449No Hit
ATTTAGAAATGTCCACTGTAGGACG33380.10269154603691542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC24350.013.8986994
TGGCGAA2100.012.66000118
GGTATCA18500.012.447871
AGGACCT39150.012.0442485
GGACCTG37750.011.9771116
GATCGTC802.87278E-511.8733177
TGTAGGA62150.011.5336482
CGAAATT752.0853136E-411.39419
GTCCTAT5100.011.3818041
GGCGAGG22700.011.29361319
CTAGGAC6500.011.261613
TAAGGCG1105.0079325E-711.22136316
CTGTAGG62200.011.1988161
GGCGAAA1701.2732926E-1111.170588519
ACCCGCG600.005884201311.0817627
GTCCTAC61250.010.90642451
TTTAGAA14000.010.8649672
GTACTAG1855.456968E-1210.8018711
GTAGGAC65200.010.7750663
TAGAAAT13250.010.7621364
Produced by FastQC (version 0.11.5)