FastQCFastQC Report
Thu 26 May 2016
SRR1512414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788138
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66870.37396442556446985No Hit
GTCCTACAGTGGACATTTCTAAATT45680.2554612675308058No Hit
GTCCTAAAGTGTGTATTTCTCATTT42580.23812479797420558No Hit
CTGTAGGACGTGGAATATGGCAAGA41640.2328679330118816No Hit
GGTATCAACGCAGAGTACTTTTTTT41310.23102243786553384No Hit
TATCAACGCAGAGTACTTTTTTTTT38250.21390966469030914No Hit
CTTTAGGACGTGAAATATGGCGAGG36430.2037314793377245No Hit
GTCCTACAGTGTGCATTTCTCATTT27740.15513344048390001No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19830.11089748106689754No Hit
CTGTAGGACCTGGAATATGGCGAGA18500.1034595763861626No Hit
CTGAAGGACCTGGAATATGGCGAGA18440.10312403181409938No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18190.10172592943050256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGG456.85226E-414.7522426
GGTATCA11200.014.369591
TAGGACC7550.013.9419684
TGCACCG500.00151968813.2766455
GTCCTAT1900.011.9522321
TAGAACG802.9197685E-511.8538134
TGGCGAA802.9450808E-511.84381818
GGCGAGG11100.011.78277819
TCGAACT1251.8973878E-811.37294219
GTGTAGG3150.011.2840961
GTCCTAG2900.011.2354561
CTCGAAC1354.9276423E-911.22969318
AAATGTC5800.011.1186857
TGTCCAC6500.011.08288710
AATGTCC6150.011.0638258
ATGTCCA6650.010.9501869
AGGACGT27550.010.9462615
TGTAGGA27050.010.9376292
GGACGTG27250.010.9278746
TTAGGAC15750.010.8979823
Produced by FastQC (version 0.11.5)