FastQCFastQC Report
Thu 26 May 2016
SRR1512414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788138
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80020.4475046109416611No Hit
GGTATCAACGCAGAGTACTTTTTTT55190.30864508220282777No Hit
TATCAACGCAGAGTACTTTTTTTTT51890.29019013073935007No Hit
CTGTAGGACGTGGAATATGGCAAGA49630.2775512851916351No Hit
GTCCTACAGTGGACATTTCTAAATT48590.27173517927587243No Hit
GTCCTAAAGTGTGTATTTCTCATTT46210.258425244584031No Hit
CTTTAGGACGTGAAATATGGCGAGG42980.24036176178796045No Hit
GTCCTACAGTGTGCATTTCTCATTT28800.1610613945903504No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26510.14825477675660378No Hit
CTGAAGGACCTGGAATATGGCGAGA23530.13158939634413003No Hit
CTGTAGGACCTGGAATATGGCGAGA21680.12124343870551378No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20660.11553918098043886No Hit
ATTTAGAAATGTCCACTGTAGGACG18820.10524914743716648No Hit
GAATATGGCAAGAAAACTGAAAATC18520.10357142457685033No Hit
GTCCTTCAGTGTGCATTTCTCATTT18220.10189370171653418No Hit
TTTCTAAATTTTCCACCTTTTTCAG18190.10172592943050256No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18140.10144630895378322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGG350.002145192816.3141861
GGTATCA12600.014.5770271
CGAGCAT400.00528405414.24815410
TAGGACC6800.013.9820794
CGAAATC1657.2759576E-1211.51172713
AATGTCC6400.011.2801048
AAATGTC6650.011.1417257
GGCGAGG10400.011.13655619
CGCCTTT1900.010.9939614
AGGACCT16600.010.940025
CGCGCCT1752.0008883E-1110.85512812
GTAGGAC25800.010.8344853
TGTAGGA26250.010.7571112
GGACGTG23600.010.7467896
GGACCTG15550.010.6291456
AGGACGT24250.010.5863165
CCACCTT7000.010.58256613
TAGAACG909.436994E-510.5642374
GCGCCTT1803.6379788E-1110.55241613
GAAATGT6800.010.4768766
Produced by FastQC (version 0.11.5)