FastQCFastQC Report
Thu 26 May 2016
SRR1512413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163135
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68280.31565297588916086No Hit
GTATCAACGCAGAGTACTTTTTTTT65320.3019691327633273No Hit
GTCCTAAAGTGTGTATTTCTCATTT64860.29984258957485316No Hit
CTGTAGGACGTGGAATATGGCAAGA62420.28856266483599036No Hit
CTTTAGGACGTGAAATATGGCGAGG54280.250932096239948No Hit
GGTATCAACGCAGAGTACTTTTTTT42480.19638164053561152No Hit
GTCCTACAGTGTGCATTTCTCATTT37520.1734519574598904No Hit
TATCAACGCAGAGTACTTTTTTTTT36860.1704008302764275No Hit
CTGAAGGACCTGGAATATGGCGAGA29660.13711580645683233No Hit
CTGTAGGACCTGGAATATGGCGAGA26900.12435654732598751No Hit
GTCCTTCAGTGTGCATTTCTCATTT26030.12033460694778643No Hit
ATTTAGAAATGTCCACTGTAGGACG24490.11321531018637303No Hit
GAATATGGCAAGAAAACTGAAAATC22640.10466290823272703No Hit
TTTCTAAATTTTCCACCTTTTTCAG22130.10230521904550571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1150.015.65762619
TAGGACC11450.013.9969694
GGTATCA12250.012.979961
GTCTTAG3000.012.5096721
TCCAACG1850.012.28989718
AAGACCG658.1444025E-411.6718455
GGCGAGG17850.011.25557819
AAATGTC9450.011.2398167
ACCTTTT8650.011.08028815
AGGACCT23800.011.077225
TAGAAAT10450.011.0712424
GTCCTAA20700.011.0688161
GGACCTG22950.010.8676596
TGGCGAG38750.010.85475418
GTGTAGG4300.010.7947591
TGTAGGA39500.010.6838032
GAAATGT10300.010.68030556
GTATCAA27200.010.6385461
ATCCTAC2600.010.6357531
AATGTCC10400.010.6324548
Produced by FastQC (version 0.11.5)