FastQCFastQC Report
Thu 26 May 2016
SRR1512413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163135
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84880.39239344747322746No Hit
GTCCTACAGTGGACATTTCTAAATT74410.34399147533556623No Hit
CTGTAGGACGTGGAATATGGCAAGA73040.33765807496989325No Hit
GTCCTAAAGTGTGTATTTCTCATTT71800.3319256542009629No Hit
CTTTAGGACGTGAAATATGGCGAGG63610.29406393960617344No Hit
GGTATCAACGCAGAGTACTTTTTTT57280.2648008561647794No Hit
TATCAACGCAGAGTACTTTTTTTTT54070.24996128304520987No Hit
GTCCTACAGTGTGCATTTCTCATTT39680.18343746460576896No Hit
CTGAAGGACCTGGAATATGGCGAGA35990.1663788898982264No Hit
CTGTAGGACCTGGAATATGGCGAGA31500.14562197921072886No Hit
ATTTAGAAATGTCCACTGTAGGACG28200.1303663432934144No Hit
GTCCTTCAGTGTGCATTTCTCATTT27780.12842471690393806No Hit
TTTCTAAATTTTCCACCTTTTTCAG27180.12565096491897176No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25780.1191788769540505No Hit
GAATATGGCAAGAAAACTGAAAATC25290.11691364616632804No Hit
GGAATATGGCGAGAAAACTGAAAAT21990.10165801024901358No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21800.10077965545377426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.014.3877421
GATCGTC400.005286299614.2474347
TAGGACC12600.013.9597834
CCCCGGT853.971718E-612.28678919
CGTGCGC802.872866E-511.87286110
GTCGAGA907.513938E-611.6041919
CTAGGAC3700.011.5634733
CCAACGA1157.136987E-811.55832319
AAGGCGT1001.9347262E-611.3979476
AATGTCC9400.011.317118
AGGCGTG1104.982612E-711.225257
TCCCGTT1104.9855953E-711.22473111
TGTCGAG2200.011.22057918
TAGAAAT10600.011.2119624
GTGGTAT4250.011.1959471
AATCCCG2800.011.18975419
GGCGAGG17450.011.04498819
GAAATGT10100.010.9089266
TTTAGAA10900.010.9046372
ACCTTTT9500.010.89425715
Produced by FastQC (version 0.11.5)