FastQCFastQC Report
Thu 26 May 2016
SRR1512412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047957
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55630.27163656268173597No Hit
GTCCTACAGTGGACATTTCTAAATT54290.2650934565520663No Hit
CTGTAGGACGTGGAATATGGCAAGA49930.243803947055529No Hit
GTCCTAAAGTGTGTATTTCTCATTT49770.24302268065198637No Hit
CTTTAGGACGTGAAATATGGCGAGG40450.19751391264562684No Hit
GGTATCAACGCAGAGTACTTTTTTT35220.17197626707982638No Hit
TATCAACGCAGAGTACTTTTTTTTT32520.15879239652004412No Hit
GTCCTACAGTGTGCATTTCTCATTT30020.14658510896469018No Hit
CTGAAGGACCTGGAATATGGCGAGA21590.10542213532803667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10850.014.2895451
CCAACGA801.3314275E-714.21236219
TAGGACC9650.013.9543384
GGACCGT550.00311031912.0693396
TCCTATA3300.011.7819752
GGCGAGG12800.011.47352119
TATACTG3200.011.2611265
GTATTAA2650.011.1795391
AGGACCT18550.011.1445055
TCCAACG1451.2805685E-911.10469718
GGACCTG17450.010.9231726
ATTTAGA8050.010.9179891
GTGTAGG3750.010.7969491
GTCCTAC26750.010.6320541
AAATGTC8300.010.625857
TGGCGAG29900.010.61205318
AATGTCC8300.010.5902148
TGTAGGA33450.010.5461752
ACGCTGT909.691307E-510.5367256
AGGACGT30300.010.5158615
Produced by FastQC (version 0.11.5)