FastQCFastQC Report
Thu 26 May 2016
SRR1512412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047957
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69530.3395090814895039No Hit
CTGTAGGACGTGGAATATGGCAAGA58510.2856993579455037No Hit
GTCCTACAGTGGACATTTCTAAATT54900.26807203471557267No Hit
GTCCTAAAGTGTGTATTTCTCATTT53260.2600640540792605No Hit
GGTATCAACGCAGAGTACTTTTTTT48110.23491704171523134No Hit
CTTTAGGACGTGAAATATGGCGAGG47280.2308642222468538No Hit
TATCAACGCAGAGTACTTTTTTTTT44380.2167037686826432No Hit
GTCCTACAGTGTGCATTTCTCATTT30550.14917305392642521No Hit
CTGAAGGACCTGGAATATGGCGAGA27230.13296177605291518No Hit
CTGTAGGACCTGGAATATGGCGAGA24670.12046151359623274No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21730.10610574343113649No Hit
GTCCTTCAGTGTGCATTTCTCATTT20710.1011251701085521No Hit
ATTTAGAAATGTCCACTGTAGGACG20600.1005880494561165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.013.9038381
ACCGCTC500.001501808213.2976748
TAGGACC9750.012.4824214
TTCCGAA701.09725355E-412.20708116
CAAGTCG1900.011.9979026
TCCAACG951.0477361E-611.99292218
AAGTCGT2150.011.9281457
ATACGGC907.514913E-611.60397815
GGCGAGG12150.011.25261819
CGAGTAA600.005884389411.08139610
AATGTCC8700.011.0268078
AAATGTC8550.010.9980777
AGGACGT29900.010.9072795
GCGATTT700.001493929910.85524511
AGTCGTC2450.010.8552458
GGACGTG30000.010.8281066
GACGTGG16200.010.7882377
CTGTAGG31150.010.7540291
TGTAGGA31750.010.6917752
CGATTTC803.7812878E-410.68458712