##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512412_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2047957 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85109062348477 34.0 31.0 34.0 30.0 34.0 2 32.019632248138024 34.0 31.0 34.0 30.0 34.0 3 32.14921016408059 34.0 31.0 34.0 30.0 34.0 4 35.59064716690829 37.0 35.0 37.0 33.0 37.0 5 35.39536328155327 37.0 35.0 37.0 33.0 37.0 6 35.42020315856241 37.0 35.0 37.0 33.0 37.0 7 35.33358610556765 37.0 35.0 37.0 33.0 37.0 8 35.29536215848282 37.0 35.0 37.0 33.0 37.0 9 36.93570128669694 39.0 37.0 39.0 33.0 39.0 10 36.864155839209516 39.0 37.0 39.0 33.0 39.0 11 36.90542672526816 39.0 37.0 39.0 33.0 39.0 12 36.82366036005639 39.0 37.0 39.0 33.0 39.0 13 36.80423758897282 39.0 37.0 39.0 33.0 39.0 14 38.12840211000524 40.0 38.0 41.0 33.0 41.0 15 38.1285705705735 40.0 38.0 41.0 33.0 41.0 16 38.08517805793774 40.0 38.0 41.0 33.0 41.0 17 38.07703823859583 40.0 38.0 41.0 33.0 41.0 18 38.02597271329427 40.0 38.0 41.0 33.0 41.0 19 38.04609715926653 40.0 38.0 41.0 33.0 41.0 20 38.02248826513447 40.0 38.0 41.0 33.0 41.0 21 37.97972516024507 40.0 38.0 41.0 33.0 41.0 22 37.921712711741506 40.0 38.0 41.0 33.0 41.0 23 37.84931275412521 40.0 38.0 41.0 33.0 41.0 24 37.79635509925257 40.0 38.0 41.0 33.0 41.0 25 37.732954354022084 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5892076436198614 1101 2 0.5837214599514908 1101 3 0.4770299533315949 1101 4 0.4690672858352798 1101 5 0.5077570676779786 1101 6 0.6010384116687462 1101 7 0.5910590789396153 1101 8 0.5775690639223612 1101 9 0.6830636648436794 1101 10 0.6947459665519915 1101 11 0.6283494683159034 1101 12 0.6841908320770358 1101 13 0.7251748463605807 1101 14 0.7986366166161076 1101 15 0.803043994320376 1101 16 0.728010988884634 1101 17 0.7146031638086043 1101 18 0.7660435549456395 1101 19 0.8398933943232478 1101 20 0.8052762880443112 1101 21 0.7576721570151079 1101 22 0.8636326125091216 1101 23 0.8662315484939995 1101 24 0.994148008509228 1101 25 0.936600215067628 1102 1 0.25440501672377636 1102 2 0.2619319338192696 1102 3 0.23135073285888552 1102 4 0.17095204257016405 1102 5 0.1502489476020017 1102 6 0.24582451744139888 1102 7 0.21696165301306536 1102 8 0.14952436323191876 1102 9 0.31173998943692993 1102 10 0.2640121814165042 1102 11 0.2988485860654393 1102 12 0.28762018273319256 1102 13 0.34240551645884665 1102 14 0.490705353480692 1102 15 0.47462129332273406 1102 16 0.4689949884950195 1102 17 0.45181090663353274 1102 18 0.5166113046637335 1102 19 0.3986183082523169 1102 20 0.45854923123936686 1102 21 0.39154832726135425 1102 22 0.4961706092198881 1102 23 0.46976504591467716 1102 24 0.33259502290621157 1102 25 0.40194519185938304 1103 1 0.5157556852782612 1103 2 0.6114417871624767 1103 3 0.5404061357230532 1103 4 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-0.8518563886354826 2315 7 -0.8086728180018028 2315 8 -0.7661859215155218 2315 9 -0.8502782602846821 2315 10 -0.834149899915893 2315 11 -0.9078254059558688 2315 12 -0.9534111588762002 2315 13 -0.9663172262439943 2315 14 -1.1600155650652084 2315 15 -1.2047009230958778 2315 16 -1.247037852541098 2315 17 -1.1860124187785601 2315 18 -1.06947199669154 2315 19 -1.0875693997870712 2315 20 -1.0107231964479837 2315 21 -0.9535218779359198 2315 22 -1.0059898269162773 2315 23 -1.0464139140130655 2315 24 -1.1491279843556725 2315 25 -1.1403412621157898 2316 1 -1.162968367984842 2316 2 -1.1094181862804682 2316 3 -1.0025381739898016 2316 4 -0.8470236624416927 2316 5 -0.9142092797043802 2316 6 -0.9303528884146246 2316 7 -0.8825884797098382 2316 8 -1.0829380225373484 2316 9 -1.2231725745705049 2316 10 -1.2528774689678102 2316 11 -1.2089600774331188 2316 12 -1.2894353032922865 2316 13 -1.2398716524817885 2316 14 -1.3776010940353913 2316 15 -1.4716019408058045 2316 16 -1.531395508822044 2316 17 -1.530656225653722 2316 18 -1.7206386500519244 2316 19 -1.7005772467490488 2316 20 -1.7159774126337481 2316 21 -1.7406132062385424 2316 22 -1.8768282203144366 2316 23 -2.0210608328197424 2316 24 -1.8590509001940418 2316 25 -1.829800854547706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15753.0 3 1.0 4 315.0 5 2628.0 6 3406.0 7 2691.0 8 2136.0 9 1924.0 10 1734.0 11 1742.0 12 1538.0 13 1566.0 14 1569.0 15 1546.0 16 1559.0 17 1715.0 18 2032.0 19 2175.0 20 2796.0 21 3573.0 22 4653.0 23 5834.0 24 7302.0 25 8801.0 26 9751.0 27 11352.0 28 12941.0 29 16280.0 30 20644.0 31 26823.0 32 35345.0 33 47013.0 34 64053.0 35 87628.0 36 132865.0 37 249331.0 38 1254942.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40223578448587 19.427094898565073 13.056243831197097 25.114425485751962 2 14.857198943867445 20.577200760407628 41.51106844303462 23.05453185269031 3 18.715041380263354 25.108339677053763 30.109470071881393 26.06714887080149 4 12.411410546258905 17.3641188907824 38.60429799846675 31.62017256449195 5 12.707649559989715 38.334423459915115 36.069648822809945 12.888278157285225 6 31.28427384986155 37.67338104827976 18.265986810902376 12.776358290956319 7 27.77008509813173 31.58863358960946 22.592398851031138 18.048882461227674 8 24.584855529724344 37.829527028347044 20.69944517162561 16.886172270303007 9 26.515498262230942 14.801249532890607 20.515249137221474 38.16800306765698 10 15.06307869352762 28.843775460561403 34.953968281879796 21.139177564031176 11 33.952937866299074 21.928363405800997 25.706562144935972 18.41213658296396 12 23.7554255814885 24.465745689575165 32.2654434298565 19.513385299079836 13 29.315244190726265 22.14806370566607 25.93190636392409 22.60478573968358 14 20.311614896883658 22.934743117438167 27.9082168822493 28.84542510342888 15 22.3434994952975 32.40063074382942 23.378054158216298 21.87781560265678 16 21.35987042342379 28.096231214301863 28.976449545168443 21.567448817105905 17 21.128636030041882 29.101912603927556 28.36424486838244 21.405206497648123 18 20.925356430296503 27.007108585870913 31.393161733756653 20.67437325007593 19 23.402346826617944 26.60812702610455 28.08081419678245 21.908711950495054 20 23.613825876226894 27.142073783775732 28.80744078122734 20.43665955877003 21 22.493733999297838 26.588448878565323 27.680854627318833 23.236962494818005 22 23.03321761559606 28.63039780759733 27.72552384291622 20.610860733890387 23 21.5499553207385 28.32616349191623 28.689701308150177 21.43417987919509 24 23.104147206215757 26.587911757912885 29.344170800461143 20.963770235410216 25 22.233181653716365 27.629730507037014 28.830976431634063 21.306111407612562 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 214.0 2 407.5 3 601.0 4 601.0 5 601.0 6 1496.5 7 2392.0 8 2392.0 9 2392.0 10 3239.0 11 4086.0 12 4086.0 13 4086.0 14 6442.0 15 8798.0 16 8798.0 17 8798.0 18 16439.5 19 24081.0 20 24081.0 21 24081.0 22 39387.5 23 54694.0 24 54694.0 25 54694.0 26 80571.0 27 106448.0 28 106448.0 29 106448.0 30 137121.5 31 167795.0 32 167795.0 33 167795.0 34 188860.0 35 209925.0 36 209925.0 37 209925.0 38 222163.0 39 234401.0 40 234401.0 41 234401.0 42 245253.5 43 256106.0 44 256106.0 45 256106.0 46 262512.5 47 268919.0 48 268919.0 49 268919.0 50 255769.0 51 242619.0 52 242619.0 53 242619.0 54 212085.0 55 181551.0 56 181551.0 57 181551.0 58 154708.5 59 127866.0 60 127866.0 61 127866.0 62 105069.0 63 82272.0 64 82272.0 65 82272.0 66 62858.0 67 43444.0 68 43444.0 69 43444.0 70 31732.5 71 20021.0 72 20021.0 73 20021.0 74 13886.0 75 7751.0 76 7751.0 77 7751.0 78 5274.5 79 2798.0 80 2798.0 81 2798.0 82 1820.0 83 842.0 84 842.0 85 842.0 86 548.0 87 254.0 88 254.0 89 254.0 90 159.5 91 65.0 92 65.0 93 65.0 94 37.5 95 10.0 96 10.0 97 10.0 98 7.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16577496500170658 2 0.005273548223912904 3 0.0 4 0.001318387055978226 5 0.11343011596434886 6 0.032227239146134415 7 0.027149007523107175 8 0.02329150465561533 9 0.038672686975361296 10 0.03286201809901282 11 0.038623857825139885 12 0.03471752580742662 13 0.02641657026978594 14 0.00844744298830493 15 0.010547096447825808 16 0.0027344324123992837 17 7.812664035426525E-4 18 5.371206524355735E-4 19 0.0 20 0.0 21 0.0 22 0.007715005734983693 23 0.001318387055978226 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2047957.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.24959141799437 #Duplication Level Percentage of deduplicated Percentage of total 1 83.12041051543078 56.72934056174114 2 11.620894236745324 15.862425671391877 3 2.771098958827432 5.673791151564054 4 1.0116939498965043 2.7619079488197316 5 0.4344160953015961 1.4824360504867218 6 0.2467762721664418 1.0105427848209256 7 0.16421546735793027 0.7845346986185614 8 0.10743133324837446 0.5865715679753561 9 0.07574918449816885 0.46528658030216674 >10 0.3891750613213581 4.728783584917082 >50 0.02947105977901431 1.3798561416949546 >100 0.02366553607999965 3.401567548668759 >500 0.0026440883691696343 1.2742769952301816 >1k 0.002072393586646401 2.7066741733567734 >5k 2.858473912615725E-4 1.152004540411687 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6953 0.3395090814895039 No Hit CTGTAGGACGTGGAATATGGCAAGA 5851 0.2856993579455037 No Hit GTCCTACAGTGGACATTTCTAAATT 5490 0.26807203471557267 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5326 0.2600640540792605 No Hit GGTATCAACGCAGAGTACTTTTTTT 4811 0.23491704171523134 No Hit CTTTAGGACGTGAAATATGGCGAGG 4728 0.2308642222468538 No Hit TATCAACGCAGAGTACTTTTTTTTT 4438 0.2167037686826432 No Hit GTCCTACAGTGTGCATTTCTCATTT 3055 0.14917305392642521 No Hit CTGAAGGACCTGGAATATGGCGAGA 2723 0.13296177605291518 No Hit CTGTAGGACCTGGAATATGGCGAGA 2467 0.12046151359623274 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2173 0.10610574343113649 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2071 0.1011251701085521 No Hit ATTTAGAAATGTCCACTGTAGGACG 2060 0.1005880494561165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.882915022141578E-5 0.0 8 0.0 0.0 0.0 4.882915022141578E-5 0.0 9 0.0 0.0 0.0 4.882915022141578E-5 0.0 10 0.0 0.0 0.0 4.882915022141578E-5 0.0 11 0.0 0.0 0.0 4.882915022141578E-5 0.0 12 0.0 0.0 0.0 4.882915022141578E-5 0.0 13 0.0 0.0 0.0 4.882915022141578E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1410 0.0 13.903838 1 ACCGCTC 50 0.0015018082 13.297674 8 TAGGACC 975 0.0 12.482421 4 TTCCGAA 70 1.09725355E-4 12.207081 16 CAAGTCG 190 0.0 11.997902 6 TCCAACG 95 1.0477361E-6 11.992922 18 AAGTCGT 215 0.0 11.928145 7 ATACGGC 90 7.514913E-6 11.603978 15 GGCGAGG 1215 0.0 11.252618 19 CGAGTAA 60 0.0058843894 11.081396 10 AATGTCC 870 0.0 11.026807 8 AAATGTC 855 0.0 10.998077 7 AGGACGT 2990 0.0 10.907279 5 GCGATTT 70 0.0014939299 10.855245 11 AGTCGTC 245 0.0 10.855245 8 GGACGTG 3000 0.0 10.828106 6 GACGTGG 1620 0.0 10.788237 7 CTGTAGG 3115 0.0 10.754029 1 TGTAGGA 3175 0.0 10.691775 2 CGATTTC 80 3.7812878E-4 10.684587 12 >>END_MODULE