FastQCFastQC Report
Thu 26 May 2016
SRR1512411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931936
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73470.3802921007735246No Hit
GTCCTACAGTGGACATTTCTAAATT50750.26268986136186706No Hit
GGTATCAACGCAGAGTACTTTTTTT45540.23572209431368327No Hit
CTGTAGGACGTGGAATATGGCAAGA44270.22914837758600698No Hit
GTCCTAAAGTGTGTATTTCTCATTT43960.22754376956586553No Hit
TATCAACGCAGAGTACTTTTTTTTT39700.20549334967617977No Hit
CTTTAGGACGTGAAATATGGCGAGG36490.18887789243536018No Hit
GTCCTACAGTGTGCATTTCTCATTT34800.1801301906481374No Hit
CTGTAGGACCTGGAATATGGCGAGA24250.12552175641429114No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20490.10605941397644643No Hit
CTGAAGGACCTGGAATATGGCGAGA19450.10067621287661703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11100.013.7573184
GGTATCA13050.013.245461
GGCGTGC655.7268415E-513.0855228
GTCGAGG751.518828E-512.63397819
CGTGCGC550.00312703412.06064710
GTATTAG1251.0299118E-811.8527941
TGTAGGA33650.011.7538972
GGTTATA1352.566594E-911.7064641
GGCGAGG10800.011.58114619
GGACCTG19250.011.381836
AGGACCT19800.011.3051845
GTGGTAT4200.011.2883751
GTAGGAC33500.011.2119523
CTGTAGG31100.011.1477241
CTGGACG600.00594669211.0656684
GACGTGG14150.010.9930527
GATCTAA906.407806E-510.9748091
TCGAACT1303.3936885E-810.9332519
GTGTAGG3350.010.9092881
GGACGGG700.001511658810.8398386
Produced by FastQC (version 0.11.5)