FastQCFastQC Report
Thu 26 May 2016
SRR1512411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931936
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88630.45876260911334543No Hit
GGTATCAACGCAGAGTACTTTTTTT60180.3115010021035894No Hit
TATCAACGCAGAGTACTTTTTTTTT55980.2897611515081245No Hit
GTCCTACAGTGGACATTTCTAAATT53740.27816656452387656No Hit
CTGTAGGACGTGGAATATGGCAAGA51240.2652261772646713No Hit
GTCCTAAAGTGTGTATTTCTCATTT47170.24415922680668511No Hit
CTTTAGGACGTGAAATATGGCGAGG43750.2264567770360923No Hit
GTCCTACAGTGTGCATTTCTCATTT37150.19229415467179037No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28190.14591580673479868No Hit
CTGTAGGACCTGGAATATGGCGAGA27700.14337949083199444No Hit
CTGAAGGACCTGGAATATGGCGAGA23550.12189844798171368No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19870.10285019793616353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.013.9922461
GCCGGTT500.001501981313.29740811
TAGGACC10150.013.2057564
CCGGTTT604.100453E-412.66387112
TACACCG701.0842017E-412.2226645
AATCCCG1405.2750693E-1112.20842819
CACCTTT7550.012.07430314
GTATTAG1501.2732926E-1112.0503321
GTACCGT658.029373E-411.6915436
TGGCGAA907.5030202E-611.60554418
CTAGGAC2800.011.5433283
AGGCGTG1406.82121E-1011.5340637
CGAAATC1406.8394E-1011.53227213
TAGGACT4300.011.274884
TGTAGGA31100.011.1574562
ATAGGAC3000.011.090653
ACCTTTT8050.011.08842315
ACACCGT951.3615438E-510.999286
GGCGTGC951.36271665E-510.9984268
AGTCGTC2600.010.959978