FastQCFastQC Report
Thu 26 May 2016
SRR1512410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110837
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT82590.2654912488182441No Hit
CTGTAGGACGTGGAATATGGCAAGA77070.24774682826519037No Hit
GTATCAACGCAGAGTACTTTTTTTT74160.2383924326475479No Hit
GTCCTAAAGTGTGTATTTCTCATTT66850.21489393369051482No Hit
CTTTAGGACGTGAAATATGGCGAGG57660.18535204512483297No Hit
GTCCTACAGTGTGCATTTCTCATTT51880.16677183664717887No Hit
GGTATCAACGCAGAGTACTTTTTTT45530.14635932387328554No Hit
TATCAACGCAGAGTACTTTTTTTTT42610.13697278256623538No Hit
CTGTAGGACCTGGAATATGGCGAGA34000.10929534398620049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC16750.013.7021764
GGCGAGG18850.012.6178819
GGTATCA16450.012.1222191
CCGACCA803.0061727E-511.820729
GTCCTAG5700.011.7768921
GCGCATA600.00441515611.5171081
GTGGTAT6100.011.3283021
AGGACCT29250.011.31588655
TCCAACG2850.011.30120618
GCGTGCG1105.277052E-711.1759549
GGACCTG28750.011.1168336
GTCCTAC43150.010.9356281
TGGCGAG45750.010.93285418
GTACTAG2450.010.8791331
TATACCG700.001513326410.8387945
TGTAGGA47400.010.7844852
TAGAAAT11800.010.7699034
GTAGGAC46500.010.7688673
AGGACGT45300.010.719165
GGACGTG45250.010.6681286
Produced by FastQC (version 0.11.5)