FastQCFastQC Report
Thu 26 May 2016
SRR1512410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110837
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93820.30159085802309793No Hit
CTGTAGGACGTGGAATATGGCAAGA87730.28201413317380497No Hit
GTCCTACAGTGGACATTTCTAAATT87480.28121049093861233No Hit
GTCCTAAAGTGTGTATTTCTCATTT72580.23331341372113037No Hit
CTTTAGGACGTGAAATATGGCGAGG67640.21743344315372357No Hit
GGTATCAACGCAGAGTACTTTTTTT62910.20222853206387864No Hit
TATCAACGCAGAGTACTTTTTTTTT60250.19367777868142882No Hit
GTCCTACAGTGTGCATTTCTCATTT55230.1775406425987604No Hit
CTGTAGGACCTGGAATATGGCGAGA40100.1289042145249012No Hit
CTGAAGGACCTGGAATATGGCGAGA37750.12134997751409027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC16000.013.6666274
GGTATCA18750.013.4988721
TCCAACG2550.012.6599918
TAGCCCG550.00305185212.1002915
GGCGAGG19100.011.98059319
GGACCGT1052.7291753E-711.7598656
CCTACGA657.9731666E-411.7017572
CCAACGA1751.8189894E-1211.39399119
AGGACCT30050.011.3582245
TGTAGGA48650.011.0808912
TTAGGAC26300.010.8808853
AGGACGT44550.010.8625195
GGACGTG44100.010.8121826
GGACCTG29700.010.745616
TGGTATC5650.010.6014812
TGGCGAG45100.010.568706518
CTGTAGG47800.010.5503061
GTAGGAC48900.010.5376543
GACGTGA22700.010.4605727
TCCTACA47850.010.3720122
Produced by FastQC (version 0.11.5)