FastQCFastQC Report
Thu 26 May 2016
SRR1512409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293834
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70490.30730209771064515No Hit
CTGTAGGACGTGGAATATGGCAAGA65150.2840222962951984No Hit
GTATCAACGCAGAGTACTTTTTTTT65070.28367353522530403No Hit
GTCCTAAAGTGTGTATTTCTCATTT63510.2768726943623645No Hit
CTTTAGGACGTGAAATATGGCGAGG54420.23724471779562076No Hit
GGTATCAACGCAGAGTACTTTTTTT42430.18497415244520746No Hit
TATCAACGCAGAGTACTTTTTTTTT40300.17568838895927083No Hit
GTCCTACAGTGTGCATTTCTCATTT34900.15214701674140324No Hit
CTGAAGGACCTGGAATATGGCGAGA28210.12298187227148957No Hit
GTCCTTCAGTGTGCATTTCTCATTT26010.11339094284939538No Hit
CTGTAGGACCTGGAATATGGCGAGA24980.10890064407450582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG602.609309E-514.2266655
TGCGATA707.4455565E-613.53940710
CTGGTCG500.001553011713.2387419
TAGGACC11400.013.0616234
GGTATCA12800.012.9648691
TCCCGCC1550.012.85129913
CGGTTTC1457.2759576E-1212.42925313
ATAGGAC3000.011.6972243
CCGCCCT1850.011.28424115
GTGTAGG5000.011.2609131
GTCCTAC32750.011.129711
GGCGAGG17200.011.07312419
GCGATAA951.3962732E-510.97452611
GTATTAT2800.010.9363511
GTCCCGC2000.010.90113412
TGTCCCG2000.010.89970111
TGTAGGA39650.010.7400282
GTCCTAT2950.010.7151131
TCCTACA37500.010.6729262
TTAGGAC22900.010.56104853
Produced by FastQC (version 0.11.5)