FastQCFastQC Report
Thu 26 May 2016
SRR1512409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293834
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84170.3669402406625763No Hit
CTGTAGGACGTGGAATATGGCAAGA75240.32800978623562127No Hit
GTCCTACAGTGGACATTTCTAAATT75090.32735585922956933No Hit
GTCCTAAAGTGTGTATTTCTCATTT67060.2923489668389256No Hit
CTTTAGGACGTGAAATATGGCGAGG63000.27464934254178813No Hit
GGTATCAACGCAGAGTACTTTTTTT56590.24670486181650458No Hit
TATCAACGCAGAGTACTTTTTTTTT55770.24313006085008768No Hit
GTCCTACAGTGTGCATTTCTCATTT37750.1645716298563889No Hit
CTGAAGGACCTGGAATATGGCGAGA35040.15275734861371834No Hit
CTGTAGGACCTGGAATATGGCGAGA30450.13274718222853094No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27570.1201917837123349No Hit
GTCCTTCAGTGTGCATTTCTCATTT26470.11539631900128781No Hit
ATTTAGAAATGTCCACTGTAGGACG25980.11326015744818502No Hit
TTTCTAAATTTTCCACCTTTTTCAG25190.10981614188297845No Hit
GAATATGGCAAGAAAACTGAAAATC23900.10419236963093231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.014.1787411
TAGGACC11550.013.3347784
ACGAAAT2700.013.015038512
CGAAATC2750.012.43276813
CGGTTTC550.003075865612.08741213
TCGTGCA658.039587E-411.6899469
ATCGTGC752.0785313E-411.39769658
AGGACCT23100.011.2769425
ATATCCG600.005844040311.0917365
TTAACGT600.005844040311.0917364
GGACCTG22050.011.0708086
GTCCTAG2800.010.8748681
CACCTTT8750.010.8521114
TCCGTAA700.00149819710.85163819
CTTTTTC10050.010.77065617
ATAGGAC3900.010.7260753
TAGAAAT10750.010.7011314
ACCTTTT8850.010.62219115
ATTTAGA10700.010.582671
GGCGAGG17550.010.44199619
Produced by FastQC (version 0.11.5)