FastQCFastQC Report
Thu 26 May 2016
SRR1512408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2495074
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87360.3501298959469739No Hit
GTCCTACAGTGGACATTTCTAAATT72420.2902519123681302No Hit
CTGTAGGACGTGGAATATGGCAAGA66100.2649220023133583No Hit
GTCCTAAAGTGTGTATTTCTCATTT63410.2541407589514379No Hit
CTTTAGGACGTGAAATATGGCGAGG54210.21726810507423827No Hit
GGTATCAACGCAGAGTACTTTTTTT53860.21586534106804045No Hit
TATCAACGCAGAGTACTTTTTTTTT50540.20255912249496408No Hit
GTCCTACAGTGTGCATTTCTCATTT41820.16761025925483572No Hit
CTGAAGGACCTGGAATATGGCGAGA28620.11470601673537538No Hit
GTACTGGTTCACTATCGGTCAGTCA28340.11358380553041714No Hit
CTGTAGGACCTGGAATATGGCGAGA25980.10412516823148331No Hit
GTCCTTCAGTGTGCATTTCTCATTT25350.10160019302032726No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25320.1014799561055103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11500.014.0184864
GGTATCA16250.013.6680821
GCCGTTC500.001555690813.2357888
GCGCCAC1155.456968E-912.37174513
GGACCGT951.0629592E-611.9788866
TCCAACG1701.8189894E-1211.70285318
AGGACCG1157.255039E-811.54486755
AAGGCGT1105.086622E-711.2075186
AGGACCT24800.011.20404155
TGTAGGA42850.011.1984632
TACACCG600.005954872411.0638315
CCGAACA600.005955765511.0636084
ACCGAGT600.006092864611.0298248
GTAGGAC42800.010.9899743
GCCGGTT1303.367677E-810.93938411
CCACCTT9050.010.89989413
AGGACGT40900.010.8744755
GGACGTG40300.010.87165456
GGACCTG24000.010.7872356
ACCTTTT10050.010.76279715
Produced by FastQC (version 0.11.5)