FastQCFastQC Report
Thu 26 May 2016
SRR1512408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2495074
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110970.44475634790791774No Hit
CTGTAGGACGTGGAATATGGCAAGA78510.3146600060759721No Hit
GTCCTACAGTGGACATTTCTAAATT78180.3133374000129856No Hit
GGTATCAACGCAGAGTACTTTTTTT72710.2914142025446941No Hit
TATCAACGCAGAGTACTTTTTTTTT72110.28900946424835494No Hit
GTCCTAAAGTGTGTATTTCTCATTT66560.2667656350072182No Hit
CTTTAGGACGTGAAATATGGCGAGG64420.25818873508360873No Hit
GTCCTACAGTGTGCATTTCTCATTT46250.18536524367613946No Hit
CTGAAGGACCTGGAATATGGCGAGA35640.14284145480254293No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34610.13871332072716078No Hit
CTGTAGGACCTGGAATATGGCGAGA31800.12745112970597267No Hit
GTACTGGTTCACTATCGGTCAGTCA29970.12011667790213837No Hit
ATTTAGAAATGTCCACTGTAGGACG26500.1062092747549772No Hit
GTCCTTCAGTGTGCATTTCTCATTT25680.10292279908331377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG350.002159319416.2985294
TAGGACC13350.014.3146274
GCGAACT400.00529209114.24520513
GGTATCA19100.014.146991
GCGCCAC802.008359E-613.058104513
AGGACCT24150.012.1650885
GGACCTG23900.011.6450916
GGACCGT907.475168E-611.6097636
ATAGGAC2950.011.6023433
AATGTCC10300.011.4355668
TGTAGGA43600.011.2953512
CCCCGTA1604.5474735E-1111.27519315
GACCGTT600.005882426611.0820477
ACCGTCC600.005882426611.0820478
GTCCTAT3450.011.0311291
CTGTAGG43800.010.9480171
GGCGAGG16350.010.85939319
GTAGGAC42750.010.8529783
AAATGTC10600.010.84308057
CCCGTAC1855.456968E-1210.77777816
Produced by FastQC (version 0.11.5)