FastQCFastQC Report
Thu 26 May 2016
SRR1512407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934647
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80840.2754675434558228No Hit
CTGTAGGACGTGGAATATGGCAAGA72980.24868408363936104No Hit
GTATCAACGCAGAGTACTTTTTTTT71190.24258454253612105No Hit
GTCCTAAAGTGTGTATTTCTCATTT70730.2410170626995342No Hit
CTTTAGGACGTGAAATATGGCGAGG57320.1953216178981663No Hit
GGTATCAACGCAGAGTACTTTTTTT42660.1453667170191168No Hit
TATCAACGCAGAGTACTTTTTTTTT40300.13732486394445398No Hit
GTCCTACAGTGTGCATTTCTCATTT38120.1298963725449773No Hit
CTGAAGGACCTGGAATATGGCGAGA31310.10669085583376808No Hit
GTCCTTCAGTGTGCATTTCTCATTT29380.10011425564982773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.014.2623321
AACACCG701.10993264E-412.19243055
GTCGAGG1251.4697434E-912.12951219
GTACTAG1403.5288394E-1012.0014491
TGTCGAG1900.011.96704518
TATGTCG1750.011.92782416
GTCCTAG5850.011.65751651
GGCGAGG18400.011.58772219
ATGTCGA2050.011.57117917
TAGGACC11950.011.4268244
ACCGAGC700.001556822610.8019918
GTCCTAA21900.010.7861531
TTAGGAC25750.010.6798853
TGTAGGA40150.010.6757572
GTCCTAC39750.010.6419061
AGGACCT25500.010.6358365
ACCGCCC803.9545563E-410.633218
GGACGTG42250.010.5493186
GACGTGA22250.010.4849367
AGGACGT43300.010.4466315
Produced by FastQC (version 0.11.5)