FastQCFastQC Report
Thu 26 May 2016
SRR1512407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934647
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA87590.2984686062753033No Hit
GTCCTACAGTGGACATTTCTAAATT84290.28722364223022395No Hit
GTATCAACGCAGAGTACTTTTTTTT82250.28027220991144763No Hit
GTCCTAAAGTGTGTATTTCTCATTT72130.2457876535065376No Hit
CTTTAGGACGTGAAATATGGCGAGG69420.23655315273012392No Hit
TATCAACGCAGAGTACTTTTTTTTT53390.18192988798993542No Hit
GGTATCAACGCAGAGTACTTTTTTT53180.18121429936888492No Hit
GTCCTACAGTGTGCATTTCTCATTT41080.13998276453692726No Hit
CTGAAGGACCTGGAATATGGCGAGA39280.1338491477850658No Hit
CTGTAGGACCTGGAATATGGCGAGA33440.11394896899013748No Hit
GTCCTTCAGTGTGCATTTCTCATTT30030.10232917281022215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.013.0611391
TAGGACC12850.013.0214994
GTCGTAC550.00307377212.08869658
TGTAGGA43500.011.1244872
CGTCTAG600.005808561611.1009831
AGGACCT27500.011.0974655
ACCGACC600.00588577811.0813058
GTCCTAA20150.010.8137191
GTAGGAC43200.010.7175783
CTGTAGG43050.010.6755091
AGGACGT44550.010.6275475
GGACCTG27050.010.569416
ATGTCGA2250.010.55056717
GGCGAGG18950.010.42251219
GGACGTG43450.010.4056386
GACGTGG22500.010.3003377
AATGTCC9600.010.28978358
ATTTAGA10250.010.2113571
GTCCTAC38950.010.2113561
TGGCGAG42400.010.14496718
Produced by FastQC (version 0.11.5)