FastQCFastQC Report
Thu 26 May 2016
SRR1512406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149796
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66410.3089130317481287No Hit
GTCCTACAGTGGACATTTCTAAATT55510.2582105464890622No Hit
GTCCTAAAGTGTGTATTTCTCATTT54650.2542101669181634No Hit
CTGTAGGACGTGGAATATGGCAAGA52710.24518605486288No Hit
CTTTAGGACGTGAAATATGGCGAGG46120.21453198349982977No Hit
GGTATCAACGCAGAGTACTTTTTTT42820.19918168979754358No Hit
TATCAACGCAGAGTACTTTTTTTTT39720.18476171692569898No Hit
GTCCTACAGTGTGCATTTCTCATTT29780.13852477165275215No Hit
CTGAAGGACCTGGAATATGGCGAGA24050.11187107986060073No Hit
CTGTAGGACCTGGAATATGGCGAGA21850.10163755072574328No Hit
ATTTAGAAATGTCCACTGTAGGACG21550.10024206948008091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.014.1650071
TAGGACC8650.013.4859114
GGACCTG18350.011.3701926
TCGCGCG1201.3616045E-711.03136359
GGCGAGG13100.010.92375419
AGGACCT19450.010.8736815
GTATCAA27300.010.5635091
GTCCTAA17300.010.5612081
AGCGACT1803.6379788E-1110.54369415
TGTAGGA34300.010.53472
CGATTTC909.7257885E-510.5330912
TTAACCC1659.567884E-1010.3461764
CTGTAGG31500.010.2837451
TGGCGAG32200.010.2397218
TATCGCG1303.9340193E-710.2132947
CCAACGA1303.973364E-710.20589219
ACCTTTT8650.010.202394515
TTTAGAA8750.010.188522
TTGGCGA750.002672560310.12218317
TAGAACG750.002685563410.11626154
Produced by FastQC (version 0.11.5)