##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512406_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2149796 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.79537825914645 31.0 23.0 33.0 10.0 34.0 2 27.51323800025677 31.0 26.0 34.0 16.0 34.0 3 28.683694173772768 31.0 28.0 34.0 16.0 34.0 4 32.48037023047768 35.0 33.0 37.0 23.0 37.0 5 32.776031772317005 35.0 35.0 37.0 26.0 37.0 6 33.02844269874909 37.0 35.0 37.0 26.0 37.0 7 32.92115484445966 37.0 35.0 37.0 26.0 37.0 8 32.93177213093708 37.0 35.0 37.0 26.0 37.0 9 34.376473395615214 39.0 35.0 39.0 26.0 39.0 10 34.29731890839875 39.0 35.0 39.0 25.0 39.0 11 34.33811487229486 39.0 35.0 39.0 26.0 39.0 12 34.244565996029394 39.0 35.0 39.0 25.0 39.0 13 34.1916074827565 39.0 35.0 39.0 25.0 39.0 14 35.223724018465006 40.0 36.0 41.0 25.0 41.0 15 35.15947234063139 40.0 36.0 41.0 24.0 41.0 16 35.017261172688016 40.0 36.0 41.0 22.0 41.0 17 34.95688149015069 40.0 36.0 41.0 22.0 41.0 18 34.920225919110464 40.0 36.0 41.0 21.0 41.0 19 34.927292170978085 40.0 36.0 41.0 19.0 41.0 20 34.835365774240906 40.0 35.0 41.0 18.0 41.0 21 34.71938360663058 40.0 35.0 41.0 18.0 41.0 22 34.59105794224196 40.0 35.0 41.0 18.0 41.0 23 34.424681690727866 39.0 35.0 41.0 15.0 41.0 24 34.29197979715285 39.0 34.0 41.0 9.0 41.0 25 34.147347469248245 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.144037404939095 1101 2 2.273285895765671 1101 3 1.040244823254728 1101 4 0.5584872605720648 1101 5 0.22827967912067493 1101 6 0.11744200317637166 1101 7 -0.12915152318502265 1101 8 -0.1344514413496114 1101 9 -0.2705478709612734 1101 10 -0.15906801716738528 1101 11 -0.21174417866208017 1101 12 -0.1595697592929355 1101 13 -0.012613939506515237 1101 14 -0.19222495449766086 1101 15 -0.32419248243084553 1101 16 -0.6155636246885265 1101 17 -0.6025003798822297 1101 18 -0.5522946010014422 1101 19 -0.5650940443000394 1101 20 -0.4218718652301803 1101 21 -0.6397128596238915 1101 22 -0.6695982629106254 1101 23 -0.712022038044303 1101 24 -0.5963706174740651 1101 25 -0.7825493450034244 1102 1 3.1359414218718697 1102 2 1.6072043607728013 1102 3 0.6327447082222903 1102 4 0.1873972131786985 1102 5 -7.683124129371777E-4 1102 6 -0.16288744972935376 1102 7 -0.20048083805181705 1102 8 -0.368314690785958 1102 9 -0.4407751750610345 1102 10 -0.3459474171581789 1102 11 -0.3538575546341107 1102 12 -0.44567153999509657 1102 13 -0.3110840080658477 1102 14 -0.5357210203882445 1102 15 -0.3585572733018694 1102 16 -0.4727140411059594 1102 17 -0.576279885702867 1102 18 -0.6625762004124809 1102 19 -0.700887538065281 1102 20 -0.8126232571226879 1102 21 -0.7887488877838322 1102 22 -0.8392181957316751 1102 23 -0.8846581214198181 1102 24 -0.81772984077503 1102 25 -0.91693944301106 1103 1 2.6407202528355356 1103 2 1.2034414153629385 1103 3 0.14642515407098244 1103 4 -0.02947570239089714 1103 5 -0.3909714895236931 1103 6 -0.3890349835190676 1103 7 -0.552304166119221 1103 8 -0.7658879749706173 1103 9 -0.8804364002672784 1103 10 -1.063526844927182 1103 11 -0.9537434594963159 1103 12 -1.0768612012202965 1103 13 -0.8105508327103976 1103 14 -1.2086848610061125 1103 15 -1.1834607312622012 1103 16 -1.2022186049114723 1103 17 -1.266088118105884 1103 18 -1.418678096147076 1103 19 -1.274838740452502 1103 20 -1.2764880105016658 1103 21 -1.4841258405660014 1103 22 -1.7037631316628676 1103 23 -1.5909684601946097 1103 24 -1.3839335444787366 1103 25 -1.6705707401850134 1104 1 3.7568473511568783 1104 2 1.6842134643903819 1104 3 0.6609701847131326 1104 4 0.3231455339533582 1104 5 0.14493645079071626 1104 6 0.01390599702983053 1104 7 -0.1658133587650994 1104 8 -0.28851653878622585 1104 9 -0.6114782334673876 1104 10 -0.4073750996567327 1104 11 -0.2771480691419299 1104 12 -0.4339821711110545 1104 13 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21 -0.7292877008996186 1108 22 -0.6501548478064976 1108 23 -0.5435412620040552 1108 24 -0.6597993912415276 1108 25 -0.6643119050139603 1109 1 3.292623806135026 1109 2 1.792276252738386 1109 3 0.5157873462123597 1109 4 0.2968967360373753 1109 5 -0.23879767865699364 1109 6 -0.3246267300879708 1109 7 -0.4881826207159463 1109 8 -0.5965939347374842 1109 9 -0.6145123543939945 1109 10 -0.7898287813814022 1109 11 -0.5231926040723636 1109 12 -0.6481410470559794 1109 13 -0.6137022709506681 1109 14 -0.8455761939634385 1109 15 -0.751899347543457 1109 16 -0.7771081518187799 1109 17 -0.7332882497095738 1109 18 -0.7855351181436916 1109 19 -0.8406217823776743 1109 20 -1.06838263355111 1109 21 -0.7661736879057273 1109 22 -0.9364595971639034 1109 23 -0.7777046888090098 1109 24 -0.9547010759696164 1109 25 -1.1161972677277845 1110 1 2.545861307030041 1110 2 0.653553805035461 1110 3 -0.38316426274003845 1110 4 -0.4885060579134617 1110 5 -0.5501513542436527 1110 6 -0.6602560096677053 1110 7 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-0.2646930253664408 2315 23 -0.23647253466879903 2315 24 -0.2645789585280056 2315 25 -0.1267823127586638 2316 1 3.321784782888411 2316 2 1.4449041025564426 2316 3 0.24086525214898913 2316 4 -0.8632997255075736 2316 5 -0.6645316432351933 2316 6 -0.93103002566383 2316 7 -0.8993263056106073 2316 8 -0.95852103373349 2316 9 -1.0877173134238802 2316 10 -1.0157186126093976 2316 11 -0.961897242933361 2316 12 -1.0091218812917617 2316 13 -0.8990382072652707 2316 14 -1.1588110555075133 2316 15 -1.1501846500660804 2316 16 -1.0117786627031577 2316 17 -0.9576206469118063 2316 18 -0.8892794234374577 2316 19 -1.033352223180941 2316 20 -0.893671894774414 2316 21 -0.9151128853965247 2316 22 -0.9830919627600707 2316 23 -0.9124347144060891 2316 24 -1.1739292580621807 2316 25 -1.2427010892132273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 110286.0 3 7.0 4 970.0 5 4234.0 6 5068.0 7 4529.0 8 4128.0 9 3887.0 10 3908.0 11 3932.0 12 3955.0 13 4147.0 14 4014.0 15 4012.0 16 4173.0 17 4463.0 18 5044.0 19 6158.0 20 7783.0 21 9496.0 22 11259.0 23 13359.0 24 15217.0 25 17125.0 26 19420.0 27 21668.0 28 25044.0 29 29709.0 30 36459.0 31 46506.0 32 60122.0 33 79246.0 34 109375.0 35 159947.0 36 262586.0 37 340102.0 38 708458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60683583574965 20.501037788942867 11.898163889419903 23.99396248588758 2 14.241626911343493 20.296725808293502 42.6279764054973 22.833670874865703 3 18.447609633147888 25.495879304615464 29.936992952662457 26.11951810957419 4 11.93215868474153 17.275536024679734 39.16188621038357 31.630419080195164 5 12.50244032673121 38.42879767256896 36.289836732240595 12.778925268459238 6 30.736553614251456 37.82573940847322 18.671989522084957 12.765717455190368 7 27.322723359546686 31.848990148727207 23.036452233151465 17.791834258574646 8 24.27027485831757 37.949438307048865 21.01698883329789 16.763298001335677 9 26.572662297182433 14.933676749864668 20.730089619964907 37.763571332987986 10 15.143100601601473 28.822239024012784 35.198906992766425 20.83575338161932 11 33.656777361994415 22.043789632264787 26.01358847142748 18.285844534313313 12 23.738071405826986 24.596226207498447 32.46032193742421 19.205380449250356 13 29.29196376448031 22.45346063387913 25.9251919673082 22.329383634332352 14 20.32955477556449 23.136216937619523 28.146408271698032 28.38782001511796 15 22.29556757826244 32.54661028417411 23.45339344894171 21.704428688621743 16 21.18795520093369 28.04813954445305 29.396160881616893 21.367744372996366 17 20.984407622939898 29.23165049133874 28.618476581039914 21.165465304681454 18 20.77799812644055 27.182932237112983 31.60594074087199 20.433128895574473 19 23.396262654704874 26.67907351430175 28.189479788834955 21.735184042158416 20 23.50754339479803 27.39657316747996 28.890754563236413 20.20512887448559 21 22.50555388716143 26.722171341382662 27.726021290367107 23.046253481088804 22 23.014937327075373 28.84835467237201 27.852019460904565 20.284688539648048 23 21.4872645880159 28.445446503800902 28.845398890421542 21.22189001776166 24 23.043762307857428 26.80485687886735 29.41860248070408 20.73277833257114 25 22.236383005939125 27.748444725792766 29.089253085572526 20.925919182695583 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2198.0 1 2198.0 2 1870.0 3 1542.0 4 1542.0 5 1542.0 6 2718.0 7 3894.0 8 3894.0 9 3894.0 10 4877.0 11 5860.0 12 5860.0 13 5860.0 14 8835.5 15 11811.0 16 11811.0 17 11811.0 18 20353.0 19 28895.0 20 28895.0 21 28895.0 22 45505.0 23 62115.0 24 62115.0 25 62115.0 26 90756.5 27 119398.0 28 119398.0 29 119398.0 30 153507.5 31 187617.0 32 187617.0 33 187617.0 34 206490.0 35 225363.0 36 225363.0 37 225363.0 38 236667.5 39 247972.0 40 247972.0 41 247972.0 42 256761.5 43 265551.0 44 265551.0 45 265551.0 46 270516.0 47 275481.0 48 275481.0 49 275481.0 50 259835.5 51 244190.0 52 244190.0 53 244190.0 54 213321.0 55 182452.0 56 182452.0 57 182452.0 58 154812.5 59 127173.0 60 127173.0 61 127173.0 62 104243.0 63 81313.0 64 81313.0 65 81313.0 66 62509.0 67 43705.0 68 43705.0 69 43705.0 70 32030.0 71 20355.0 72 20355.0 73 20355.0 74 14243.5 75 8132.0 76 8132.0 77 8132.0 78 5590.5 79 3049.0 80 3049.0 81 3049.0 82 2089.5 83 1130.0 84 1130.0 85 1130.0 86 749.0 87 368.0 88 368.0 89 368.0 90 253.0 91 138.0 92 138.0 93 138.0 94 94.0 95 50.0 96 50.0 97 50.0 98 47.0 99 44.0 100 44.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.3717171303695785 2 0.20378677790822944 3 0.19364628085641614 4 0.18169165818524177 5 0.16592272010925688 6 0.2014609758321255 7 0.4732542064456348 8 0.2588152550288493 9 0.23597587864150832 10 0.2103455397628426 11 0.20690335269020876 12 0.22537022117447422 13 0.24002277425392923 14 0.24779095318811645 15 0.3169603069314484 16 0.5200493442168467 17 0.5233519831649143 18 0.24258115653764356 19 0.22067210098074422 20 0.21434591933374145 21 0.24895385422616842 22 0.33696220478594247 23 0.350777469118 24 0.31891398067537574 25 0.359569000965673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2149796.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.0210892523743 #Duplication Level Percentage of deduplicated Percentage of total 1 86.39035960934169 62.219277999691094 2 9.517939503908293 13.709847410193564 3 2.1412928774194966 4.6265473632030885 4 0.7334328818689737 2.112905401828458 5 0.34968394430438465 1.2592309281434184 6 0.18962980140507227 0.8194406911142833 7 0.12996026479071546 0.6551915880828021 8 0.08693005761703979 0.500863795068069 9 0.06056750859662686 0.39259241481884494 >10 0.34874332408620484 4.5887649542090045 >50 0.02597533852070503 1.2974740219386727 >100 0.02104390351957913 3.1361388344969563 >500 0.0024164181026738256 1.2433956872185696 >1k 0.0017633321289779915 2.35988834572063 >5k 2.61234389478221E-4 1.0784405642725545 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6641 0.3089130317481287 No Hit GTCCTACAGTGGACATTTCTAAATT 5551 0.2582105464890622 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5465 0.2542101669181634 No Hit CTGTAGGACGTGGAATATGGCAAGA 5271 0.24518605486288 No Hit CTTTAGGACGTGAAATATGGCGAGG 4612 0.21453198349982977 No Hit GGTATCAACGCAGAGTACTTTTTTT 4282 0.19918168979754358 No Hit TATCAACGCAGAGTACTTTTTTTTT 3972 0.18476171692569898 No Hit GTCCTACAGTGTGCATTTCTCATTT 2978 0.13852477165275215 No Hit CTGAAGGACCTGGAATATGGCGAGA 2405 0.11187107986060073 No Hit CTGTAGGACCTGGAATATGGCGAGA 2185 0.10163755072574328 No Hit ATTTAGAAATGTCCACTGTAGGACG 2155 0.10024206948008091 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.6516041522079306E-5 0.0 6 0.0 0.0 0.0 4.6516041522079306E-5 0.0 7 0.0 0.0 0.0 4.6516041522079306E-5 0.0 8 0.0 0.0 0.0 4.6516041522079306E-5 0.0 9 0.0 0.0 0.0 4.6516041522079306E-5 0.0 10 0.0 0.0 0.0 4.6516041522079306E-5 0.0 11 0.0 0.0 0.0 4.6516041522079306E-5 0.0 12 0.0 0.0 0.0 4.6516041522079306E-5 0.0 13 0.0 0.0 0.0 4.6516041522079306E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1255 0.0 14.165007 1 TAGGACC 865 0.0 13.485911 4 GGACCTG 1835 0.0 11.370192 6 TCGCGCG 120 1.3616045E-7 11.0313635 9 GGCGAGG 1310 0.0 10.923754 19 AGGACCT 1945 0.0 10.873681 5 GTATCAA 2730 0.0 10.563509 1 GTCCTAA 1730 0.0 10.561208 1 AGCGACT 180 3.6379788E-11 10.543694 15 TGTAGGA 3430 0.0 10.5347 2 CGATTTC 90 9.7257885E-5 10.53309 12 TTAACCC 165 9.567884E-10 10.346176 4 CTGTAGG 3150 0.0 10.283745 1 TGGCGAG 3220 0.0 10.23972 18 TATCGCG 130 3.9340193E-7 10.213294 7 CCAACGA 130 3.973364E-7 10.205892 19 ACCTTTT 865 0.0 10.2023945 15 TTTAGAA 875 0.0 10.18852 2 TTGGCGA 75 0.0026725603 10.122183 17 TAGAACG 75 0.0026855634 10.1162615 4 >>END_MODULE