FastQCFastQC Report
Thu 26 May 2016
SRR1512406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149796
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81920.3810594121488737No Hit
CTGTAGGACGTGGAATATGGCAAGA61450.2858410751531773No Hit
GTCCTACAGTGGACATTTCTAAATT60170.2798870218383512No Hit
GTCCTAAAGTGTGTATTTCTCATTT57690.2683510435408755No Hit
CTTTAGGACGTGAAATATGGCGAGG57230.2662113056308599No Hit
GGTATCAACGCAGAGTACTTTTTTT56570.26314124689040264No Hit
TATCAACGCAGAGTACTTTTTTTTT55730.25923389940254793No Hit
GTCCTACAGTGTGCATTTCTCATTT32480.15108410286371357No Hit
CTGAAGGACCTGGAATATGGCGAGA30070.13987373685689247No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26630.12387221857329718No Hit
ATTTAGAAATGTCCACTGTAGGACG25930.12061609566675165No Hit
CTGTAGGACCTGGAATATGGCGAGA25550.11884848608891263No Hit
TTTCTAAATTTTCCACCTTTTTCAG23120.10754508799904734No Hit
GTCCTTCAGTGTGCATTTCTCATTT22600.10512625383989924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.013.8162721
TAGGACC9950.012.995914
GACCGTG1052.0005245E-812.6641067
TATGTCG1352.7284841E-1112.65880516
AAATGTC8650.012.6275057
GGACCGT1001.4472062E-712.3475046
AATGTCC9200.012.285568
CAACCGT550.003073752212.0884656
ATGCGCC550.00307428812.08818311
GAAATGT9750.011.6899456
GGCGAGG14950.011.62154519
CCCGTAC907.5159332E-611.60390516
TTTAGAA11350.011.4779982
TCCAACG1501.7826096E-1011.39292418
ATTTAGA10150.011.34818651
TGTCCAC10550.011.07333410
TGTCGAG1658.185452E-1110.93260418
ACCGTAT700.001494321610.8549498
TAGAAAT11000.010.718154
CACCTTT8350.010.6884714
Produced by FastQC (version 0.11.5)